5a2h

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==Crystal Structure of Arabidopsis thaliana Calmodulin-7==
==Crystal Structure of Arabidopsis thaliana Calmodulin-7==
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<StructureSection load='5a2h' size='340' side='right' caption='[[5a2h]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
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<StructureSection load='5a2h' size='340' side='right'caption='[[5a2h]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5a2h]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A2H FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5a2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A2H FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2h OCA], [http://pdbe.org/5a2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a2h RCSB], [http://www.ebi.ac.uk/pdbsum/5a2h PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2h OCA], [https://pdbe.org/5a2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a2h RCSB], [https://www.ebi.ac.uk/pdbsum/5a2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a2h ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CALM7_ARATH CALM7_ARATH] Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Activates MPK8 in vitro.<ref>PMID:21419340</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Calmodulin (CaM) is a Ca(2+) sensor that participates in several cellular signaling cascades by interacting with various targets, including DNA. It has been shown that Arabidopsis thaliana CaM7 (AtCaM7) interacts with Z-box DNA and functions as a transcription factor [Kushwaha R et al. (2008) Plant Cell 20, 1747-1759; Abbas N et al. (2014) Plant Cell 26, 1036-1052]. The crystal structure of AtCaM7, and a model of the AtCAM7-Z-box complex suggest that Arg-127 determines the DNA-binding ability by forming crucial interactions with the guanine base. We validated the model using biolayer interferometry, which confirmed that AtCaM7 interacts with Z-box DNA with high affinity. In contrast, the AtCaM2/3/5 isoform does not show any binding, although it differs from AtCaM7 by only a single residue.
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Crystal structure of Arabidopsis thaliana calmodulin7 and insight into its mode of DNA binding.,Kumar S, Mazumder M, Gupta N, Chattopadhyay S, Gourinath S FEBS Lett. 2016 Sep;590(17):3029-39. doi: 10.1002/1873-3468.12349. Epub 2016 Aug , 24. PMID:27500568<ref>PMID:27500568</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5a2h" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Calmodulin 3D structures|Calmodulin 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Gourinath, S]]
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[[Category: Arabidopsis thaliana]]
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[[Category: Kumar, S]]
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[[Category: Large Structures]]
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[[Category: Calcium-binding protein]]
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[[Category: Gourinath S]]
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[[Category: Protein]]
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[[Category: Kumar S]]

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Crystal Structure of Arabidopsis thaliana Calmodulin-7

PDB ID 5a2h

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