5esx

<StructureSection load='5esx' size='340' side='right' caption='[[5esx]], [[Resolution|resolution]] 2.71&Aring;' scene=''>

<table><tr><td colspan='2'>[[5esx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ESX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ESX FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5esw|5esw]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5esx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5esx OCA], [http://pdbe.org/5esx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5esx RCSB], [http://www.ebi.ac.uk/pdbsum/5esx PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (EC 2.4.2.8) reversibly catalyzes the transfer of the 5-phophoribosyl group from 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP) to hypoxanthine or guanine to form inosine monophosphate (IMP) or guanosine monophosphate (GMP) in the purine salvage pathway. To investigate the catalytic mechanism of this enzyme in the intracellular pathogen Legionella pneumophila, we determined the crystal structures of the L. pneumophila HGPRT (LpHGPRT) both in its apo-form and in complex with GMP. The structures reveal that LpHGPRT comprises a core domain and a hood domain which are packed together to create a cavity for GMP-binding and the enzymatic catalysis. The binding of GMP induces conformational changes of the stable loop II. This new binding site is closely related to the Gout arthritis-linked human HGPRT mutation site (Ser103Arg). Finally, these structures of LpHGPRT provide insights into the catalytic mechanism of HGPRT.

 

<div class="pdbe-citations 5esx" style="background-color:#fffaf0;"></div>

== References ==

[[Category: Chen, X]]

[[Category: Ge, H]]

[[Category: Gong, X]]

[[Category: Lu, M]]

[[Category: Qin, X]]

[[Category: Zhang, N]]

[[Category: Hypoxanthine-guanine phosphoribosyltransferase]]

[[Category: Transferase]]