5fyd

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==Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity==
==Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity==
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<StructureSection load='5fyd' size='340' side='right' caption='[[5fyd]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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<StructureSection load='5fyd' size='340' side='right'caption='[[5fyd]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5fyd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FYD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5fyd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Collinsella_aerofaciens Collinsella aerofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FYD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/7-beta-hydroxysteroid_dehydrogenase_(NADP(+)) 7-beta-hydroxysteroid dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.201 1.1.1.201] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fyd OCA], [http://pdbe.org/5fyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fyd RCSB], [http://www.ebi.ac.uk/pdbsum/5fyd PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fyd OCA], [https://pdbe.org/5fyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fyd RCSB], [https://www.ebi.ac.uk/pdbsum/5fyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fyd ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HSDHB_COLAA HSDHB_COLAA] 7beta-hydroxysteroid dehydrogenase that catalyzes the reduction of the 7-oxo group of 7-oxo-lithocholate (7-oxo-LCA), to yield ursodeoxycholate (UDCA) (PubMed:21181147, PubMed:6954878, PubMed:27006087). As C.aerofaciens is an intestinal bacterium, this enzyme probably contributes to the formation of UDCA in the human colon. UDCA is regarded as a chemopreventive beneficial secondary bile acid due to its low hydrophobicity; it protects hepatocytes and bile duct epithelial cells against necrosis and apoptosis induced by more hydrophobic secondary bile acids like deoxycholate (DCA) (Probable). This enzyme is also able to catalyze the reverse reaction, i.e. the oxidation of the 7beta-hydroxy group of UDCA to 7-oxo-LCA (PubMed:21181147, PubMed:6954878). To a lesser extent, is also active on the taurine- and glycine-conjugates of ursodeoxycholate. It is specific for NADPH/NADP(+) as the electron acceptor/donor since it is not active with NADH/NAD(+) (PubMed:6954878). In the presence of NADPH, 7beta-HSDH can also reduce dehydrocholate (PubMed:21181147). And is also able to oxidize ursocholate (PubMed:27006087).<ref>PMID:21181147</ref> <ref>PMID:27006087</ref> <ref>PMID:6954878</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 5fyd" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 5fyd" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ferrandi, E]]
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[[Category: Collinsella aerofaciens]]
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[[Category: Forneris, F]]
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[[Category: Large Structures]]
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[[Category: Mattevi, A]]
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[[Category: Ferrandi E]]
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[[Category: Monti, D]]
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[[Category: Forneris F]]
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[[Category: Riva, S]]
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[[Category: Mattevi A]]
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[[Category: Rovida, S]]
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[[Category: Monti D]]
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[[Category: Savino, S]]
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[[Category: Riva S]]
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[[Category: Dehydrogenase]]
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[[Category: Rovida S]]
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[[Category: Oxidoreductase]]
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[[Category: Savino S]]
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[[Category: Short-chain dehydrogenase]]
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[[Category: Stereoselectivity]]
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[[Category: Steroid]]
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Current revision

Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity

PDB ID 5fyd

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