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4ufq
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==Structure of a novel Hyaluronidase (Hyal_Sk) from Streptomyces koganeiensis.== | ==Structure of a novel Hyaluronidase (Hyal_Sk) from Streptomyces koganeiensis.== | ||
| - | <StructureSection load='4ufq' size='340' side='right' caption='[[4ufq]], [[Resolution|resolution]] 1.45Å' scene=''> | + | <StructureSection load='4ufq' size='340' side='right'caption='[[4ufq]], [[Resolution|resolution]] 1.45Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4ufq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFQ OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[4ufq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_koganeiensis Streptomyces koganeiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UFQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ufq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufq OCA], [https://pdbe.org/4ufq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ufq RCSB], [https://www.ebi.ac.uk/pdbsum/4ufq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ufq ProSAT]</span></td></tr> | |
| - | + | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0U2E2J7_9ACTN A0A0U2E2J7_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hyaluronidases (Hyals) are broadly used in medical applications to facilitate the dispersion and/or absorption of fluids or medications. This study reports the isolation, cloning, and industrial-scale recombinant production, purification and full characterization, including X-ray structure determination at 1.45 A, of an extracellular Hyal from the nonpathogenic bacterium Streptomyces koganeiensis. The recombinant S. koganeiensis Hyal (rHyal_Sk) has a novel bacterial catalytic domain with high enzymatic activity, compared with commercially available Hyals, and is more thermostable and presents higher proteolytic resistance, with activity over a broad pH range. Moreover, rHyal_Sk exhibits remarkable substrate specificity for hyaluronic acid (HA) and poses no risk of animal cross-infection. | ||
| + | |||
| + | Identification and characterization of a bacterial hyaluronidase and its production in recombinant form.,Messina L, Gavira JA, Pernagallo S, Unciti-Broceta JD, Sanchez Martin RM, Diaz-Mochon JJ, Vaccaro S, Conejero-Muriel M, Pineda-Molina E, Caruso S, Musumeci L, Di Pasquale R, Pontillo A, Sincinelli F, Pavan M, Secchieri C FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12258. PMID:27311405<ref>PMID:27311405</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4ufq" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Hyaluronidase 3D structures|Hyaluronidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Bisicchia | + | [[Category: Streptomyces koganeiensis]] |
| - | [[Category: Caruso | + | [[Category: Bisicchia S]] |
| - | [[Category: Conejero-Muriel | + | [[Category: Caruso S]] |
| - | [[Category: Diaz-Mochon | + | [[Category: Conejero-Muriel M]] |
| - | [[Category: Gavira | + | [[Category: Di Pasquale R]] |
| - | [[Category: Messina | + | [[Category: Diaz-Mochon JJ]] |
| - | [[Category: Musumeci | + | [[Category: Gavira JA]] |
| - | [[Category: | + | [[Category: Messina L]] |
| - | [[Category: Unciti-Broceta | + | [[Category: Musumeci L]] |
| - | [[Category: Vaccaro | + | [[Category: Pernagallo S]] |
| + | [[Category: Unciti-Broceta JD]] | ||
| + | [[Category: Vaccaro S]] | ||
Current revision
Structure of a novel Hyaluronidase (Hyal_Sk) from Streptomyces koganeiensis.
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