5dm9

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==XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA==
==XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA==
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<StructureSection load='5dm9' size='340' side='right' caption='[[5dm9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='5dm9' size='340' side='right'caption='[[5dm9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5dm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DM9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5dm9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DM9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm9 OCA], [http://pdbe.org/5dm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dm9 RCSB], [http://www.ebi.ac.uk/pdbsum/5dm9 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm9 OCA], [https://pdbe.org/5dm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dm9 RCSB], [https://www.ebi.ac.uk/pdbsum/5dm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dm9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 microm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 A from &lt;0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.
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Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography.,Mafune F, Miyajima K, Tono K, Takeda Y, Kohno JY, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S, Yabashi M Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):520-3. doi:, 10.1107/S2059798316001480. Epub 2016 Mar 24. PMID:27050131<ref>PMID:27050131</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5dm9" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Kobayashi, J]]
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[[Category: Kobayashi J]]
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[[Category: Nango, E]]
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[[Category: Nango E]]
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[[Category: Hydrolase]]
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[[Category: Serial femtosecond crystallography]]
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XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA

PDB ID 5dm9

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