5ikl

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==Crystal structure of P. aeruginosa geranyl-CoA carboxylase (GCC), beta subunit==
==Crystal structure of P. aeruginosa geranyl-CoA carboxylase (GCC), beta subunit==
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<StructureSection load='5ikl' size='340' side='right' caption='[[5ikl]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='5ikl' size='340' side='right'caption='[[5ikl]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ikl]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IKL FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ikl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IKL FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ikl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikl OCA], [http://pdbe.org/5ikl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikl RCSB], [http://www.ebi.ac.uk/pdbsum/5ikl PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ikl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikl OCA], [https://pdbe.org/5ikl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ikl RCSB], [https://www.ebi.ac.uk/pdbsum/5ikl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikl ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9HZV9_PSEAE Q9HZV9_PSEAE]
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Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3-methylcrotonyl-CoA carboxylase (MCC) and propionyl-CoA carboxylase (PCC). Here we report the crystal structure of the 750-kDa alpha6beta6 holoenzyme of GCC, which is similar to MCC but strikingly different from PCC. The structures provide evidence in support of two distinct lineages of biotin-dependent acyl-CoA carboxylases, one carboxylating the alpha carbon of a saturated organic acid and the other carboxylating the gamma carbon of an alpha-beta unsaturated acid. Structural differences in the active site region of GCC and MCC explain their distinct substrate preferences. Especially, a glycine residue in GCC is replaced by phenylalanine in MCC, which blocks access by the larger geranyl-CoA substrate. Mutation of this residue in the two enzymes can change their substrate preferences.
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Structure and substrate selectivity of the 750-kDa alpha6beta6 holoenzyme of geranyl-CoA carboxylase.,Jurado AR, Huang CS, Zhang X, Zhou ZH, Tong L Nat Commun. 2015 Nov 23;6:8986. doi: 10.1038/ncomms9986. PMID:26593090<ref>PMID:26593090</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5ikl" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Huang, C S]]
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[[Category: Large Structures]]
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[[Category: Jurado, A R]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Tong, L]]
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[[Category: Huang CS]]
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[[Category: Carboxylase]]
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[[Category: Jurado AR]]
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[[Category: Ligase]]
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[[Category: Tong L]]
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[[Category: Organic acid metabolism]]
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Current revision

Crystal structure of P. aeruginosa geranyl-CoA carboxylase (GCC), beta subunit

PDB ID 5ikl

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