5i9s
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==MicroED structure of proteinase K at 1.75 A resolution== | |
| + | <StructureSection load='5i9s' size='340' side='right'caption='[[5i9s]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5i9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I9S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 1.75Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i9s OCA], [https://pdbe.org/5i9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i9s RCSB], [https://www.ebi.ac.uk/pdbsum/5i9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i9s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images. | ||
| - | + | Modeling truncated pixel values of faint reflections in MicroED images.,Hattne J, Shi D, de la Cruz MJ, Reyes FE, Gonen T J Appl Crystallogr. 2016 May 11;49(Pt 3):1029-1034. eCollection 2016 Jun 1. PMID:27275145<ref>PMID:27275145</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5i9s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Parengyodontium album]] | ||
| + | [[Category: Gonen T]] | ||
| + | [[Category: Hattne J]] | ||
| + | [[Category: Reyes FE]] | ||
| + | [[Category: Shi D]] | ||
| + | [[Category: De la Cruz MJ]] | ||
Current revision
MicroED structure of proteinase K at 1.75 A resolution
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