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5jpq
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5jpq is ON HOLD Authors: Turk, M., Cheng, J., Berninghausen, O., Kornprobst, M., Flemming, D., Kos-Braun, I.C., Kos, M., Thoms, M., Hurt, E., Beckma...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of the 90S pre-ribosome== | |
| + | <SX load='5jpq' size='340' side='right' viewer='molstar' caption='[[5jpq]], [[Resolution|resolution]] 7.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5jpq]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JPQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.3Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [https://pdbe.org/5jpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [https://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G0S5L1_CHATD G0S5L1_CHATD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of approximately 70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 beta-propellers and large alpha-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding. | ||
| - | + | Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.,Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870<ref>PMID:27419870</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[ | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5jpq" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: Beckmann | + | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Flemming | + | __TOC__ |
| - | [[Category: | + | </SX> |
| - | [[Category: Kornprobst | + | [[Category: Chaetomium thermophilum]] |
| - | [[Category: Kos-Braun | + | [[Category: Large Structures]] |
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Beckmann R]] | ||
| + | [[Category: Berninghausen O]] | ||
| + | [[Category: Cheng J]] | ||
| + | [[Category: Flemming D]] | ||
| + | [[Category: Hurt E]] | ||
| + | [[Category: Kornprobst M]] | ||
| + | [[Category: Kos M]] | ||
| + | [[Category: Kos-Braun IC]] | ||
| + | [[Category: Thoms M]] | ||
| + | [[Category: Turk M]] | ||
Current revision
Cryo-EM structure of the 90S pre-ribosome
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