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| - | [[Image:1iml.gif|left|200px]] | |
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| - | {{Structure
| + | ==CYSTEINE RICH INTESTINAL PROTEIN, NMR, 48 STRUCTURES== |
| - | |PDB= 1iml |SIZE=350|CAPTION= <scene name='initialview01'>1iml</scene>
| + | <StructureSection load='1iml' size='340' side='right'caption='[[1iml]]' scene=''> |
| - | |SITE= | + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | + | <table><tr><td colspan='2'>[[1iml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IML FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE= CRIP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10117 Rattus rattus])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iml OCA], [https://pdbe.org/1iml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iml RCSB], [https://www.ebi.ac.uk/pdbsum/1iml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iml ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iml OCA], [http://www.ebi.ac.uk/pdbsum/1iml PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1iml RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/CRIP1_RAT CRIP1_RAT] Seems to have a role in zinc absorption and may function as an intracellular zinc transport protein. |
| - | | + | == Evolutionary Conservation == |
| - | '''CYSTEINE RICH INTESTINAL PROTEIN, NMR, 48 STRUCTURES'''
| + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | | + | Check<jmol> |
| - | | + | <jmolCheckbox> |
| - | ==Overview== | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/1iml_consurf.spt"</scriptWhenChecked> |
| - | LIM domains are Zn-binding arrays found in a number of proteins involved in the control of cell differentiation, including several developmentally regulated transcription factors and a human proto-oncogene product. The rat cysteine-rich intestinal protein, CRIP, is a 76-residue polypeptide which contains a LIM motif. The solution structure of CRIP has been determined by homonuclear and 1H-15N heteronuclear correlated nuclear magnetic resonance spectroscopy. Structures with individual distance violations of < or = 0.03 angstrom and penalties (squared sum of distance violations) of < or = 0.06 angstrom2 were generated with a total of 500 nuclear Overhauser effect (NOE)-derived distance restraints (averaging 15.6 restraints per refined residue). Superposition of backbone heavy atoms of ordered residues relative to mean atom positions is achieved with pairwise rms deviations of 0.54(+/-0.14) angstrom. As observed previously for a peptide with the sequence of the C-terminal LIM domain from the avian cysteine-rich protein, CRP (cCRP-LIM2), CRIP binds two equivalents of zinc, forming N-terminal CCHC (Cys3, Cys6, His24, Cys27) and C-terminal CCCC (Cys30, Cys33, Cys51, Cys55) modules. The CCHC and CCCC modules in CRIP contain two orthogonally-arrayed antiparallel beta-sheets. The C-terminal end of the CCHC module contains a tight turn and the C terminus of the CCCC module forms an alpha-helix. The modules pack via hydrophobic interactions, forming a compact structure that is similar to that observed for cCRP-LIM2. The most significant differences between the structures occur at the CCHC module-CCCC module interface, which results in a difference in the relative orientations of the modules, and at the C terminus where the alpha-helix appears to be packed more tightly against the preceding antiparallel beta-sheet. The greater abundance of NOE information obtained for CRIP relative to cCRP-LIM2, combined with the analysis of J-coupling and proton chemical shift data, have allowed a more detailed evaluation of the molecular level interactions that stabilize the fold of the LIM motif.
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==About this Structure== | + | </jmolCheckbox> |
| - | 1IML is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IML OCA].
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iml ConSurf]. |
| - | | + | <div style="clear:both"></div> |
| - | ==Reference==
| + | __TOC__ |
| - | Structure of the cysteine-rich intestinal protein, CRIP., Perez-Alvarado GC, Kosa JL, Louis HA, Beckerle MC, Winge DR, Summers MF, J Mol Biol. 1996 Mar 22;257(1):153-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8632452 8632452]
| + | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Rattus rattus]] | | [[Category: Rattus rattus]] |
| - | [[Category: Single protein]]
| + | [[Category: Beckerle MC]] |
| - | [[Category: Beckerle, M C.]] | + | [[Category: Kosa JL]] |
| - | [[Category: Kosa, J L.]] | + | [[Category: Louis HA]] |
| - | [[Category: Louis, H A.]] | + | [[Category: Perez-Alvarado GC]] |
| - | [[Category: Perez-Alvarado, G C.]] | + | [[Category: Summers MF]] |
| - | [[Category: Summers, M F.]] | + | [[Category: Winge DR]] |
| - | [[Category: Winge, D R.]] | + | |
| - | [[Category: lim domain protein]]
| + | |
| - | [[Category: metal-binding protein]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:21:47 2008''
| + | |
