5l3r
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5l3r is ON HOLD Authors: Bange, G., Kribelbauer, J., Wild, K., Sinning, I. Description: Structure of the GTPase heterodimer of chloroplast SRP54 an...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the GTPase heterodimer of chloroplast SRP54 and FtsY from Arabidopsis thaliana== | |
| + | <StructureSection load='5l3r' size='340' side='right'caption='[[5l3r]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5l3r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L3R FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l3r OCA], [https://pdbe.org/5l3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l3r RCSB], [https://www.ebi.ac.uk/pdbsum/5l3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l3r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SR54C_ARATH SR54C_ARATH] Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.<ref>PMID:10480939</ref> <ref>PMID:11356852</ref> <ref>PMID:17475780</ref> <ref>PMID:18633119</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The signal recognition particle (SRP) is a ribonucleoprotein complex with a key role in targeting and insertion of membrane proteins. The two SRP GTPases, SRP54 (Ffh in bacteria) and FtsY (SRalpha in eukaryotes), form the core of the targeting complex (TC) regulating the SRP cycle. The architecture of the TC and its stimulation by RNA has been described for the bacterial SRP system while this information is lacking for other domains of life. Here, we present the crystal structures of the GTPase heterodimers of archaeal (Sulfolobus solfataricus), eukaryotic (Homo sapiens), and chloroplast (Arabidopsis thaliana) SRP systems. The comprehensive structural comparison combined with Brownian dynamics simulations of TC formation allows for the description of the general blueprint and of specific adaptations of the quasi-symmetric heterodimer. Our work defines conserved external nucleotide-binding sites for SRP GTPase activation by RNA. Structural analyses of the GDP-bound, post-hydrolysis states reveal a conserved, magnesium-sensitive switch within the I-box. Overall, we provide a general model for SRP cycle regulation by RNA. | ||
| - | + | Structural Basis for Conserved Regulation and Adaptation of the Signal Recognition Particle Targeting Complex.,Wild K, Bange G, Motiejunas D, Kribelbauer J, Hendricks A, Segnitz B, Wade RC, Sinning I J Mol Biol. 2016 May 27. pii: S0022-2836(16)30184-X. doi:, 10.1016/j.jmb.2016.05.015. PMID:27241309<ref>PMID:27241309</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Bange | + | <div class="pdbe-citations 5l3r" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kribelbauer | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]] |
| + | *[[Signal recognition particle receptor 3D structures|Signal recognition particle receptor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bange G]] | ||
| + | [[Category: Kribelbauer J]] | ||
| + | [[Category: Sinning I]] | ||
| + | [[Category: Wild K]] | ||
Current revision
Structure of the GTPase heterodimer of chloroplast SRP54 and FtsY from Arabidopsis thaliana
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