5exy

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of in cellulo recombinant CPV1 Polyhedra

Structural highlights

5exy is a 1 chain structure with sequence from Bombyx mori cypovirus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.55Å
Ligands:	, , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYHD_CPVBM Major component of the virus occlusion bodies, which are large proteinaceous structures (polyhedra), that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.

Publication Abstract from PubMed

While structure determination from micrometre-sized crystals used to represent a challenge, serial X-ray crystallography on microfocus beamlines at synchrotron and free-electron laser facilities greatly facilitates this process today for microcrystals and nanocrystals. In addition to typical microcrystals of purified recombinant protein, these advances have enabled the analysis of microcrystals produced inside living cells. Here, a pipeline where crystals are grown in insect cells, sorted by flow cytometry and directly analysed by X-ray diffraction is presented and applied to in vivo-grown crystals of the recombinant CPV1 polyhedrin. When compared with the analysis of purified crystals, in cellulo diffraction produces data of better quality and a gain of approximately 0.35 A in resolution for comparable beamtime usage. Importantly, crystals within cells are readily derivatized with gold and iodine compounds through the cellular membrane. Using the multiple isomorphous replacement method, a near-complete model was autobuilt from 2.7 A resolution data. Thus, in favourable cases, an in cellulo pipeline can replace the complete workflow of structure determination without compromising the quality of the resulting model. In addition to its efficiency, this approach maintains the protein in a cellular context throughout the analysis, which reduces the risk of disrupting transient or labile interactions in protein-protein or protein-ligand complexes.

A pipeline for structure determination of in vivo-grown crystals using in cellulo diffraction.,Boudes M, Garriga D, Fryga A, Caradoc-Davies T, Coulibaly F Acta Crystallogr D Struct Biol. 2016 Apr 1;72(Pt 4):576-85. doi:, 10.1107/S2059798316002369. Epub 2016 Mar 30. PMID:27050136^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Boudes M, Garriga D, Fryga A, Caradoc-Davies T, Coulibaly F. A pipeline for structure determination of in vivo-grown crystals using in cellulo diffraction. Acta Crystallogr D Struct Biol. 2016 Apr 1;72(Pt 4):576-85. doi:, 10.1107/S2059798316002369. Epub 2016 Mar 30. PMID:27050136 doi:http://dx.doi.org/10.1107/S2059798316002369

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5exy"

Categories: Bombyx mori cypovirus 1 | Large Structures | Boudes M | Coulibaly F | Garriga D

@@ Line 1: / Line 1: @@
 ==Crystal structure of in cellulo recombinant CPV1 Polyhedra==
-<StructureSection load='5exy' size='340' side='right' caption='[[5exy]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
+<StructureSection load='5exy' size='340' side='right'caption='[[5exy]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5exy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EXY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EXY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5exy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EXY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5exz|5exz]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5exy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5exy OCA], [https://pdbe.org/5exy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5exy RCSB], [https://www.ebi.ac.uk/pdbsum/5exy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5exy ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5exy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5exy OCA], [http://pdbe.org/5exy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5exy RCSB], [http://www.ebi.ac.uk/pdbsum/5exy PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PYHD_CPVBM PYHD_CPVBM]] Major component of the virus occlusion bodies, which are large proteinaceous structures (polyhedra), that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
+[https://www.uniprot.org/uniprot/PYHD_CPVBM PYHD_CPVBM] Major component of the virus occlusion bodies, which are large proteinaceous structures (polyhedra), that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 19: @@
 </div>
 <div class="pdbe-citations 5exy" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Polyhedrin|Polyhedrin]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Boudes, M]]
+[[Category: Bombyx mori cypovirus 1]]
-[[Category: Coulibaly, F]]
+[[Category: Large Structures]]
-[[Category: Garriga, D]]
+[[Category: Boudes M]]
-[[Category: In cellulo data collection]]
+[[Category: Coulibaly F]]
-[[Category: In vivo crystal]]
+[[Category: Garriga D]]
-[[Category: Viral protein]]

5exy

From Proteopedia

Current revision

Crystal structure of in cellulo recombinant CPV1 Polyhedra

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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