5i1t
From Proteopedia
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==2.6 Angstrom Resolution Crystal Structure of Stage II Sporulation Protein D (SpoIID) from Clostridium difficile in Complex with Triacetylchitotriose== | ==2.6 Angstrom Resolution Crystal Structure of Stage II Sporulation Protein D (SpoIID) from Clostridium difficile in Complex with Triacetylchitotriose== | ||
| - | <StructureSection load='5i1t' size='340' side='right' caption='[[5i1t]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='5i1t' size='340' side='right'caption='[[5i1t]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5i1t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I1T OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5i1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_630 Clostridioides difficile 630]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I1T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand= | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900017:triacetyl-beta-chitotriose'>PRD_900017</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i1t OCA], [https://pdbe.org/5i1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i1t RCSB], [https://www.ebi.ac.uk/pdbsum/5i1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i1t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q18CL6_CLOD6 Q18CL6_CLOD6] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial spores are the most resistant form of life known on the Earth and represent a serious problem for i) bioterrorism attack, ii) horizontal transmission of microbial pathogens in the community and iii) persistence in patients and in a nosocomial environment. SpoIID (Stage II Sporulation Protein D) is a Lytic Transglycosylase (LT) essential for sporulation. The LT-superfamily is a potential drug target because they are active in essential bacterial processes involving the peptidoglycan (PGN), which is unique to bacteria. However, the absence of structural information for the sporulation specific LT enzymes has hindered mechanistic understanding of SpoIID. Here, we report the first crystal structures with and without ligands of the SpoIID family from two community relevant spore-forming pathogens, Bacillus anthracis and Clostridium difficile. The structures allow us to visualize the overall architecture, characterize the substrate recognition model, identify critical residues and provide the structural basis for catalysis by this new family of enzymes. | ||
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| + | Crystal structures of the SpoIID lytic transglycosylases essential for bacterial sporulation.,Nocadello S, Minasov G, Shuvalova LS, Dubrovska I, Sabini E, Anderson WF J Biol Chem. 2016 May 18. pii: jbc.M116.729749. PMID:27226615<ref>PMID:27226615</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5i1t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Clostridioides difficile 630]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Dubrovska | + | [[Category: Anderson WF]] |
| - | [[Category: Grimshaw | + | [[Category: Dubrovska I]] |
| - | [[Category: Kwon | + | [[Category: Grimshaw S]] |
| - | [[Category: Minasov | + | [[Category: Kwon K]] |
| - | [[Category: Nocadello | + | [[Category: Minasov G]] |
| - | [[Category: Shuvalova | + | [[Category: Nocadello S]] |
| - | + | [[Category: Shuvalova L]] | |
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Current revision
2.6 Angstrom Resolution Crystal Structure of Stage II Sporulation Protein D (SpoIID) from Clostridium difficile in Complex with Triacetylchitotriose
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