1j3p

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[[Image:1j3p.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of Thermococcus litoralis phosphoglucose isomerase==
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|PDB= 1j3p |SIZE=350|CAPTION= <scene name='initialview01'>1j3p</scene>, resolution 2.02&Aring;
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<StructureSection load='1j3p' size='340' side='right'caption='[[1j3p]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>
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<table><tr><td colspan='2'>[[1j3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J3P FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3p OCA], [https://pdbe.org/1j3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j3p RCSB], [https://www.ebi.ac.uk/pdbsum/1j3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j3p ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1j3q|1J3Q]], [[1j3r|1J3R]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3p OCA], [http://www.ebi.ac.uk/pdbsum/1j3p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j3p RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/G6PI_THELI G6PI_THELI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j3/1j3p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j3p ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Thermococcus litoralis phosphoglucose isomerase'''
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==See Also==
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*[[Phosphoglucose isomerase 3D structures|Phosphoglucose isomerase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The gene encoding phosphoglucose isomerase was cloned from Thermococcus litoralis, and functionally expressed in Escherichia coli. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in k(cat). The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.
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[[Category: Large Structures]]
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==About this Structure==
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1J3P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3P OCA].
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==Reference==
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Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis., Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T, FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12560104 12560104]
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[[Category: Glucose-6-phosphate isomerase]]
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[[Category: Single protein]]
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[[Category: Thermococcus litoralis]]
[[Category: Thermococcus litoralis]]
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[[Category: Fushinobu, S.]]
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[[Category: Fushinobu S]]
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[[Category: Hidaka, M.]]
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[[Category: Hidaka M]]
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[[Category: Ito, S.]]
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[[Category: Ito S]]
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[[Category: Jeong, J J.]]
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[[Category: Jeong J-J]]
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[[Category: Shoun, H.]]
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[[Category: Shoun H]]
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[[Category: Wakagi, T.]]
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[[Category: Wakagi T]]
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[[Category: archaea]]
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[[Category: cupin superfamily]]
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[[Category: iron ion]]
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[[Category: phosphoglucose isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:28:26 2008''
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Current revision

Crystal structure of Thermococcus litoralis phosphoglucose isomerase

PDB ID 1j3p

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