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| | ==NMR structure of the HLTF HIRAN domain== | | ==NMR structure of the HLTF HIRAN domain== |
| - | <StructureSection load='5k5f' size='340' side='right' caption='[[5k5f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5k5f' size='340' side='right'caption='[[5k5f]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5k5f]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2l1i 2l1i]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K5F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5k5f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2l1i 2l1i]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K5F FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2mzn|2mzn]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5f OCA], [https://pdbe.org/5k5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k5f RCSB], [https://www.ebi.ac.uk/pdbsum/5k5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5f ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5f OCA], [http://pdbe.org/5k5f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k5f RCSB], [http://www.ebi.ac.uk/pdbsum/5k5f PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HLTF_HUMAN HLTF_HUMAN]] Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.<ref>PMID:10391891</ref> <ref>PMID:18316726</ref> <ref>PMID:18719106</ref> <ref>PMID:7876228</ref> <ref>PMID:8672239</ref> <ref>PMID:9126292</ref> | + | [https://www.uniprot.org/uniprot/HLTF_HUMAN HLTF_HUMAN] Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.<ref>PMID:10391891</ref> <ref>PMID:18316726</ref> <ref>PMID:18719106</ref> <ref>PMID:7876228</ref> <ref>PMID:8672239</ref> <ref>PMID:9126292</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arrowsmith, C]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Bezsonova, I]] | + | [[Category: Large Structures]] |
| - | [[Category: Bountra, C]] | + | [[Category: Arrowsmith C]] |
| - | [[Category: Dhe-Paganon, S]] | + | [[Category: Bezsonova I]] |
| - | [[Category: Edwards, A]] | + | [[Category: Bountra C]] |
| - | [[Category: Korzhnev, D]] | + | [[Category: Dhe-Paganon S]] |
| - | [[Category: Neculai, D]] | + | [[Category: Edwards A]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Korzhnev D]] |
| - | [[Category: Weigelt, J]] | + | [[Category: Neculai D]] |
| - | [[Category: Dna binding protein]]
| + | [[Category: Weigelt J]] |
| - | [[Category: Dna replication]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Sgc]]
| + | |
| Structural highlights
Function
HLTF_HUMAN Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.[1] [2] [3] [4] [5] [6]
References
- ↑ Ding H, Benotmane AM, Suske G, Collen D, Belayew A. Functional interactions between Sp1 or Sp3 and the helicase-like transcription factor mediate basal expression from the human plasminogen activator inhibitor-1 gene. J Biol Chem. 1999 Jul 9;274(28):19573-80. PMID:10391891
- ↑ Unk I, Hajdu I, Fatyol K, Hurwitz J, Yoon JH, Prakash L, Prakash S, Haracska L. Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3768-73. doi:, 10.1073/pnas.0800563105. Epub 2008 Mar 3. PMID:18316726 doi:http://dx.doi.org/10.1073/pnas.0800563105
- ↑ Motegi A, Liaw HJ, Lee KY, Roest HP, Maas A, Wu X, Moinova H, Markowitz SD, Ding H, Hoeijmakers JH, Myung K. Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks. Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12411-6. Epub 2008 Aug 21. PMID:18719106 doi:0805685105
- ↑ Sheridan PL, Schorpp M, Voz ML, Jones KA. Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter. J Biol Chem. 1995 Mar 3;270(9):4575-87. PMID:7876228
- ↑ Ding H, Descheemaeker K, Marynen P, Nelles L, Carvalho T, Carmo-Fonseca M, Collen D, Belayew A. Characterization of a helicase-like transcription factor involved in the expression of the human plasminogen activator inhibitor-1 gene. DNA Cell Biol. 1996 Jun;15(6):429-42. PMID:8672239
- ↑ Gong X, Kaushal S, Ceccarelli E, Bogdanova N, Neville C, Nguyen T, Clark H, Khatib ZA, Valentine M, Look AT, Rosenthal N. Developmental regulation of Zbu1, a DNA-binding member of the SWI2/SNF2 family. Dev Biol. 1997 Mar 15;183(2):166-82. PMID:9126292 doi:http://dx.doi.org/10.1006/dbio.1996.8486
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