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4zuh

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==Complex structure of PEDV 3CLpro mutant (C144A) with a peptide substrate.==
==Complex structure of PEDV 3CLpro mutant (C144A) with a peptide substrate.==
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<StructureSection load='4zuh' size='340' side='right' caption='[[4zuh]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
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<StructureSection load='4zuh' size='340' side='right'caption='[[4zuh]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4zuh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZUH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4zuh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_epidemic_diarrhea_virus Porcine epidemic diarrhea virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZUH FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zuh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zuh OCA], [http://pdbe.org/4zuh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zuh RCSB], [http://www.ebi.ac.uk/pdbsum/4zuh PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.394&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zuh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zuh OCA], [https://pdbe.org/4zuh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zuh RCSB], [https://www.ebi.ac.uk/pdbsum/4zuh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zuh ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/R4JK63_9ALPC R4JK63_9ALPC]
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Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here, we present the crystal structures of PEDV 3CL(pro) and a molecular complex between an inactive PEDV 3CL(pro) variant C144A bound to a peptide substrate. Structural characterization, mutagenesis and biochemical analysis reveal the substrate-binding pockets and the residues that comprise the active site of PEDV 3CL(pro). The dimerization of PEDV 3CL(pro) is similar to that of other Alphacoronavirus 3CL(pro)s but has several differences from that of SARS-CoV 3CL(pro) from the genus Betacoronavirus. Furthermore, the non-conserved motifs in the pockets cause different cleavage of substrate between PEDV and SARS-CoV 3CL(pro)s, which may provide new insights into the recognition of substrates by 3CL(pro)s in various coronavirus genera.
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Structural basis for the dimerization and substrate recognition specificity of porcine epidemic diarrhea virus 3C-like protease.,Ye G, Deng F, Shen Z, Luo R, Zhao L, Xiao S, Fu ZF, Peng G Virology. 2016 Jul;494:225-35. doi: 10.1016/j.virol.2016.04.018. Epub 2016 Apr, 26. PMID:27128350<ref>PMID:27128350</ref>
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==See Also==
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*[[Virus protease 3D structures|Virus protease 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4zuh" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fu, Z F]]
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[[Category: Large Structures]]
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[[Category: Peng, G Q]]
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[[Category: Porcine epidemic diarrhea virus]]
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[[Category: Ye, G]]
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[[Category: Synthetic construct]]
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[[Category: 3c-like protease]]
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[[Category: Fu ZF]]
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[[Category: Hydrolase-peptide complex]]
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[[Category: Peng GQ]]
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[[Category: Ye G]]

Current revision

Complex structure of PEDV 3CLpro mutant (C144A) with a peptide substrate.

PDB ID 4zuh

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