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1jat

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[[Image:1jat.gif|left|200px]]
 
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{{Structure
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==Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex==
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|PDB= 1jat |SIZE=350|CAPTION= <scene name='initialview01'>1jat</scene>, resolution 1.6&Aring;
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<StructureSection load='1jat' size='340' side='right'caption='[[1jat]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JAT FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [https://pdbe.org/1jat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [https://www.ebi.ac.uk/pdbsum/1jat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jat ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB]</span>
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[https://www.uniprot.org/uniprot/UBC13_YEAST UBC13_YEAST] Has a role in the DNA error-free postreplication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jat_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jat ConSurf].
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<div style="clear:both"></div>
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'''Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex'''
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==See Also==
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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__TOC__
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==Overview==
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</StructureSection>
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While the signaling properties of ubiquitin depend on the topology of polyubiquitin chains, little is known concerning the molecular basis of specificity in chain assembly and recognition. UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and I kappa B alpha kinase activation. The crystal structure of the Mms2/Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites for the two substrate ubiquitins. Mutations that destabilize the heterodimer interface confer a marked UV sensitivity, providing direct evidence that the intact heterodimer is necessary for DNA repair. Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63-linked polyubiquitin signals.
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[[Category: Large Structures]]
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==About this Structure==
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1JAT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA].
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==Reference==
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Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer., VanDemark AP, Hofmann RM, Tsui C, Pickart CM, Wolberger C, Cell. 2001 Jun 15;105(6):711-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11440714 11440714]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Hofmann RM]]
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[[Category: Hofmann, R M.]]
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[[Category: Pickart CM]]
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[[Category: Pickart, C M.]]
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[[Category: Tsui C]]
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[[Category: Tsui, C.]]
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[[Category: VanDemark AP]]
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[[Category: VanDemark, A P.]]
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[[Category: Wolberger C]]
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[[Category: Wolberger, C.]]
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[[Category: e2]]
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[[Category: ubiquitin]]
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[[Category: ubiquitin-conjugating enzyme]]
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[[Category: uev]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:30:57 2008''
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Current revision

Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex

PDB ID 1jat

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