5k5o

From Proteopedia

(Difference between revisions)

Current revision

Structure of AspA-26mer DNA complex

Structural highlights

5k5o is a 6 chain structure with sequence from Sulfolobus sp. NOB8H2 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O93706_9CREN

Publication Abstract from PubMed

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.,Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D. Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages. Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031 doi:http://dx.doi.org/10.1126/science.aaa9046

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5k5o"

Categories: Large Structures | Sulfolobus sp. NOB8H2 | Synthetic construct | Schumacher M

@@ Line 1: / Line 1: @@
 ==Structure of AspA-26mer DNA complex==
-<StructureSection load='5k5o' size='340' side='right' caption='[[5k5o]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+<StructureSection load='5k5o' size='340' side='right'caption='[[5k5o]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5k5o]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K5O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5k5o]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_sp._NOB8H2 Sulfolobus sp. NOB8H2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K5O FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5o OCA], [http://pdbe.org/5k5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k5o RCSB], [http://www.ebi.ac.uk/pdbsum/5k5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5o ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5o OCA], [https://pdbe.org/5k5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k5o RCSB], [https://www.ebi.ac.uk/pdbsum/5k5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5o ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O93706_9CREN O93706_9CREN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Schumacher, M]]
+[[Category: Large Structures]]
-[[Category: Archaea]]
+[[Category: Sulfolobus sp. NOB8H2]]
-[[Category: Aspa]]
+[[Category: Synthetic construct]]
-[[Category: Centromere dna]]
+[[Category: Schumacher M]]
-[[Category: Dna segregation]]
-[[Category: Pnob8]]
-[[Category: Transcription-dna complex]]

5k5o

From Proteopedia

Current revision

Structure of AspA-26mer DNA complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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