1jl5

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[[Image:1jl5.jpg|left|200px]]
 
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{{Structure
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==Novel Molecular Architecture of YopM-a Leucine-rich Effector Protein from Yersinia pestis==
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|PDB= 1jl5 |SIZE=350|CAPTION= <scene name='initialview01'>1jl5</scene>, resolution 2.10&Aring;
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<StructureSection load='1jl5' size='340' side='right'caption='[[1jl5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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<table><tr><td colspan='2'>[[1jl5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JL5 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE= YopM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl5 OCA], [https://pdbe.org/1jl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jl5 RCSB], [https://www.ebi.ac.uk/pdbsum/1jl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jl5 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1g9u|1G9U]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl5 OCA], [http://www.ebi.ac.uk/pdbsum/1jl5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jl5 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/YOPM_YERPE YOPM_YERPE] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jl5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jl5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many Gram-negative bacterial pathogens employ a contact-dependent (type III) secretion system to deliver effector proteins into the cytosol of animal or plant cells. Collectively, these effectors enable the bacteria to evade the immune response of the infected organism by modulating host-cell functions. YopM, a member of the leucine-rich repeat protein superfamily, is an effector produced by the bubonic plague bacterium, Yersinia pestis, that is essential for virulence. Here, we report crystal structures of YopM at 2.4 and 2.1 A resolution. Among all leucine-rich repeat family members whose atomic coordinates have been reported, the repeating unit of YopM has the least canonical secondary structure. In both crystals, four YopM monomers form a hollow cylinder with an inner diameter of 35 A. The domain that targets YopM for translocation into eukaryotic cells adopts a well-ordered, alpha-helical conformation that packs tightly against the proximal leucine-rich repeat module. A similar alpha-helical domain can be identified in virulence-associated leucine-rich repeat proteins produced by Salmonella typhimurium and Shigella flexneri, and in the conceptual translation products of several open reading frames in Y. pestis.
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'''Novel Molecular Architecture of YopM-a Leucine-rich Effector Protein from Yersinia pestis'''
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Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit.,Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS J Mol Biol. 2001 Sep 28;312(4):807-21. PMID:11575934<ref>PMID:11575934</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1jl5" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Many Gram-negative bacterial pathogens employ a contact-dependent (type III) secretion system to deliver effector proteins into the cytosol of animal or plant cells. Collectively, these effectors enable the bacteria to evade the immune response of the infected organism by modulating host-cell functions. YopM, a member of the leucine-rich repeat protein superfamily, is an effector produced by the bubonic plague bacterium, Yersinia pestis, that is essential for virulence. Here, we report crystal structures of YopM at 2.4 and 2.1 A resolution. Among all leucine-rich repeat family members whose atomic coordinates have been reported, the repeating unit of YopM has the least canonical secondary structure. In both crystals, four YopM monomers form a hollow cylinder with an inner diameter of 35 A. The domain that targets YopM for translocation into eukaryotic cells adopts a well-ordered, alpha-helical conformation that packs tightly against the proximal leucine-rich repeat module. A similar alpha-helical domain can be identified in virulence-associated leucine-rich repeat proteins produced by Salmonella typhimurium and Shigella flexneri, and in the conceptual translation products of several open reading frames in Y. pestis.
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1JL5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL5 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit., Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS, J Mol Biol. 2001 Sep 28;312(4):807-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11575934 11575934]
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[[Category: Single protein]]
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[[Category: Yersinia pestis]]
[[Category: Yersinia pestis]]
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[[Category: Anderson, D E.]]
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[[Category: Anderson DE]]
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[[Category: Evdokimov, A G.]]
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[[Category: Evdokimov AG]]
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[[Category: Routzahn, K M.]]
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[[Category: Routzahn KM]]
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[[Category: Waugh, D S.]]
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[[Category: Waugh DS]]
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[[Category: effector protein]]
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[[Category: leucine-rich repeat]]
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[[Category: molecular pathogenesis]]
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[[Category: virulence factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:35:13 2008''
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Current revision

Novel Molecular Architecture of YopM-a Leucine-rich Effector Protein from Yersinia pestis

PDB ID 1jl5

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