1jnr

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[[Image:1jnr.jpg|left|200px]]
 
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{{Structure
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==Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution==
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|PDB= 1jnr |SIZE=350|CAPTION= <scene name='initialview01'>1jnr</scene>, resolution 1.60&Aring;
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<StructureSection load='1jnr' size='340' side='right'caption='[[1jnr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
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<table><tr><td colspan='2'>[[1jnr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JNR FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase Adenylyl-sulfate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.99.2 1.8.99.2] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jnr OCA], [https://pdbe.org/1jnr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jnr RCSB], [https://www.ebi.ac.uk/pdbsum/1jnr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jnr ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jnr OCA], [http://www.ebi.ac.uk/pdbsum/1jnr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jnr RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/O28603_ARCFU O28603_ARCFU]
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== Evolutionary Conservation ==
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'''Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/1jnr_consurf.spt"</scriptWhenChecked>
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The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5'-phosphosulfate, APS) reductase catalyzes reversibly the reduction of APS to sulfite and AMP. The structures of APS reductase from the hyperthermophilic Archaeoglobus fulgidus in the two-electron reduced state and with sulfite bound to FAD are reported at 1.6- and 2.5- resolution, respectively. The FAD-sulfite adduct was detected after soaking the crystals with APS. This finding and the architecture of the active site strongly suggest that catalysis involves a nucleophilic attack of the N5 atom of reduced FAD on the sulfur atom of APS. In view of the high degree of similarity between APS reductase and fumarate reductase especially with regard to the FAD-binding alpha-subunit, it is proposed that both subunits originate from a common ancestor resembling archaeal APS reductase. The two electrons required for APS reduction are transferred via two [4Fe-4S] clusters from the surface of the protein to FAD. The exceptionally large difference in reduction potential of these clusters (-60 and -500 mV) can be explained by interactions of the clusters with the protein matrix.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1JNR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNR OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jnr ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution., Fritz G, Roth A, Schiffer A, Buchert T, Bourenkov G, Bartunik HD, Huber H, Stetter KO, Kroneck PM, Ermler U, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1836-41. Epub 2002 Feb 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11842205 11842205]
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</StructureSection>
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[[Category: Adenylyl-sulfate reductase]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Archaeoglobus fulgidus]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Bartunik HD]]
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[[Category: Bartunik, H D.]]
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[[Category: Bourenkov G]]
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[[Category: Bourenkov, G.]]
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[[Category: Buechert T]]
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[[Category: Buechert, T.]]
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[[Category: Ermler U]]
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[[Category: Ermler, U.]]
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[[Category: Fritz G]]
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[[Category: Fritz, G.]]
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[[Category: Huber H]]
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[[Category: Huber, H.]]
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[[Category: Kroneck PMH]]
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[[Category: Kroneck, P M.H.]]
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[[Category: Roth A]]
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[[Category: Roth, A.]]
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[[Category: Schiffer A]]
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[[Category: Schiffer, A.]]
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[[Category: Stetter KO]]
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[[Category: Stetter, K O.]]
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[[Category: sulfur metabolism/ adenylylsulfate reductase/ iron-sulfur flavoprotein/ crystal structure/catalysis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:36:17 2008''
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Current revision

Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution

PDB ID 1jnr

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