5k58

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==Structure of the K. pneumonia SlmA-DNA complex bound to the C-terminal of the cell division protein FtsZ==
==Structure of the K. pneumonia SlmA-DNA complex bound to the C-terminal of the cell division protein FtsZ==
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<StructureSection load='5k58' size='340' side='right' caption='[[5k58]], [[Resolution|resolution]] 2.77&Aring;' scene=''>
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<StructureSection load='5k58' size='340' side='right'caption='[[5k58]], [[Resolution|resolution]] 2.77&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5k58]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K58 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5k58]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O139:H28_str._E24377A Escherichia coli O139:H28 str. E24377A] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K58 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k58 OCA], [http://pdbe.org/5k58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k58 RCSB], [http://www.ebi.ac.uk/pdbsum/5k58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k58 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.772&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k58 OCA], [https://pdbe.org/5k58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k58 RCSB], [https://www.ebi.ac.uk/pdbsum/5k58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k58 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SLMA_ECO24 SLMA_ECO24]] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.
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[https://www.uniprot.org/uniprot/SLMA_ECOLI SLMA_ECOLI] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.[HAMAP-Rule:MF_01839]<ref>PMID:15916962</ref> <ref>PMID:21113127</ref> <ref>PMID:21321206</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cell division in most prokaryotes is mediated by FtsZ, which polymerizes to create the cytokinetic Z ring. Multiple FtsZ-binding proteins regulate FtsZ polymerization to ensure the proper spatiotemporal formation of the Z ring at the division site. The DNA-binding protein SlmA binds to FtsZ and prevents Z-ring formation through the nucleoid in a process called "nucleoid occlusion" (NO). As do most FtsZ-accessory proteins, SlmA interacts with the conserved C-terminal domain (CTD) that is connected to the FtsZ core by a long, flexible linker. However, SlmA is distinct from other regulatory factors in that it must be DNA-bound to interact with the FtsZ CTD. Few structures of FtsZ regulator-CTD complexes are available, but all reveal the CTD bound as a helix. To deduce the molecular basis for the unique SlmA-DNA-FtsZ CTD regulatory interaction and provide insight into FtsZ-regulator protein complex formation, we determined structures of Escherichia coli, Vibrio cholera, and Klebsiella pneumonia SlmA-DNA-FtsZ CTD ternary complexes. Strikingly, the FtsZ CTD does not interact with SlmA as a helix but binds as an extended conformation in a narrow, surface-exposed pocket formed only in the DNA-bound state of SlmA and located at the junction between the DNA-binding and C-terminal dimer domains. Binding studies are consistent with the structure and underscore key interactions in complex formation. Combined, these data reveal the molecular basis for the SlmA-DNA-FtsZ interaction with implications for SlmA's NO function and underscore the ability of the FtsZ CTD to adopt a wide range of conformations, explaining its ability to bind diverse regulatory proteins.
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Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.,Schumacher MA, Zeng W Proc Natl Acad Sci U S A. 2016 May 3;113(18):4988-93. doi:, 10.1073/pnas.1602327113. Epub 2016 Apr 18. PMID:27091999<ref>PMID:27091999</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5k58" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Schumacher, M]]
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[[Category: Escherichia coli O139:H28 str. E24377A]]
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[[Category: Dna]]
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[[Category: Large Structures]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Synthetic construct]]
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[[Category: Ftsz]]
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[[Category: Schumacher M]]
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[[Category: Nucleoid occlusion]]
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[[Category: Slma]]
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Current revision

Structure of the K. pneumonia SlmA-DNA complex bound to the C-terminal of the cell division protein FtsZ

PDB ID 5k58

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