1jr3

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[[Image:1jr3.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III==
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|PDB= 1jr3 |SIZE=350|CAPTION= <scene name='initialview01'>1jr3</scene>, resolution 2.7&Aring;
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<StructureSection load='1jr3' size='340' side='right'caption='[[1jr3]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1jr3]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JR3 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr3 OCA], [https://pdbe.org/1jr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jr3 RCSB], [https://www.ebi.ac.uk/pdbsum/1jr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jr3 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1jqj|1JQJ]], [[1jql|1JQL]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr3 OCA], [http://www.ebi.ac.uk/pdbsum/1jr3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jr3 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/DPO3X_ECOLI DPO3X_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. Isoform tau: serves as a scaffold to help in the dimerization of the core complex. Isoform gamma: seems to interact with the delta subunit. to transfer the beta subunit on the DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/1jr3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jr3 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III'''
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==See Also==
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex reveals a pentameric arrangement of subunits, with stoichiometry delta':gamma(3):delta. The C-terminal domains of the subunits form a circular collar that supports an asymmetric arrangement of the N-terminal ATP binding domains of the gamma motor and the structurally related domains of the delta' stator and the delta wrench. The structure suggests a mechanism by which the gamma complex switches between a closed state, in which the beta-interacting element of delta is hidden by delta', and an open form similar to the crystal structure, in which delta is free to bind to beta.
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==About this Structure==
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1JR3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR3 OCA].
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==Reference==
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Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III., Jeruzalmi D, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):429-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11525729 11525729]
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Donnell, M O.]]
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[[Category: Jeruzalmi D]]
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[[Category: Jeruzalmi, D.]]
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[[Category: Kuriyan J]]
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[[Category: Kuriyan, J.]]
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[[Category: O'Donnell M]]
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[[Category: aaa+ atpase]]
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[[Category: clamp loader]]
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[[Category: dna polymerase]]
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[[Category: processivity]]
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[[Category: processivity clamp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:37:45 2008''
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Current revision

Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III

PDB ID 1jr3

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