5hft

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==Crystal structure of HpxW==
==Crystal structure of HpxW==
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<StructureSection load='5hft' size='340' side='right' caption='[[5hft]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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<StructureSection load='5hft' size='340' side='right'caption='[[5hft]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5hft]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HFT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5hft]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_MGH_78578 Klebsiella pneumoniae subsp. pneumoniae MGH 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HFT FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hft OCA], [http://pdbe.org/5hft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hft RCSB], [http://www.ebi.ac.uk/pdbsum/5hft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hft ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.646&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hft OCA], [https://pdbe.org/5hft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hft RCSB], [https://www.ebi.ac.uk/pdbsum/5hft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hft ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/HPXW_KLEP7 HPXW_KLEP7] Involved in the uric acid degradation pathway. Catalyzes the conversion of oxamate to oxalate.<ref>PMID:27303801</ref>
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HpxW from the ubiquitous pathogen Klebsiella pneumoniae is involved in a novel uric acid degradation pathway downstream from the formation of oxalurate. Specifically, HpxW is an oxamate amidohydrolase which catalyzes the conversion of oxamate to oxalate and is a member of the Ntn-hydrolase superfamily. HpxW is autoprocessed from an inactive precursor to form a heterodimer, resulting in a 35.5 kDa alpha subunit and a 20 kDa beta subunit. Here, the structure of HpxW is presented and the substrate complex is modeled. In addition, the steady-state kinetics of this enzyme and two active-site variants were characterized. These structural and biochemical studies provide further insight into this class of enzymes and allow a mechanism for catalysis consistent with other members of the Ntn-hydrolase superfamily to be proposed.
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Biochemical and structural characterization of Klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway.,Hicks KA, Ealick SE Acta Crystallogr D Struct Biol. 2016 Jun 1;72(Pt 6):808-16. doi:, 10.1107/S2059798316007099. Epub 2016 May 25. PMID:27303801<ref>PMID:27303801</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5hft" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ealick, S E]]
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[[Category: Klebsiella pneumoniae subsp. pneumoniae MGH 78578]]
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[[Category: Hicks, K A]]
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[[Category: Large Structures]]
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[[Category: Amidohydrolase]]
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[[Category: Ealick SE]]
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[[Category: Transferase]]
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[[Category: Hicks KA]]

Current revision

Crystal structure of HpxW

PDB ID 5hft

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