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5g5z
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==S.pneumoniae ABC-transporter substrate binding protein FusA in complex with kestose== | |
| + | <StructureSection load='5g5z' size='340' side='right'caption='[[5g5z]], [[Resolution|resolution]] 2.01Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5g5z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G5Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900029:1-kestose'>PRD_900029</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5z OCA], [https://pdbe.org/5g5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g5z RCSB], [https://www.ebi.ac.uk/pdbsum/5g5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FUSA_STRPN FUSA_STRPN] Part of the ABC transporter complex FusABC-MsmK involved in short- and long-chain fructooligosaccharide (FOS) import. Required for the utilization of long-chain FOSs (PubMed:23264576). Binds kestose, nystose, fructofuranosyl-nystose and inulin, but not sucrose. Has a preference for long-chain FOSs (tetrasaccharides and larger) (PubMed:27939783).<ref>PMID:27939783</ref> <ref>PMID:23264576</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Streptococcus pneumoniae is dependent on carbohydrate uptake for colonization and pathogenesis, and dedicates over a third of its transport systems to their uptake. The ability of the pneumococcus to utilize fructooligosaccharides (FOSs) is attributed to the presence of one of two types of FOS ATP-binding cassette (ABC) transporters. Strains encoding SfuABC are only able to utilize short-chain FOSs, while strains encoding FusABC can utilize both short- and long-chain FOSs. The crystal structures of the substrate-binding protein FusA in its open and closed conformations bound to FOSs, and solution scattering data of SfuA, delineate the structural basis for import of short- and long-chain FOSs. The structure of FusA identifies an EF hand-like calcium-binding motif. This is shown to be essential for translocation of FOSs in FusABC and forms the basis for the definition of a new class of substrate-binding proteins that regulate substrate translocation by calcium. | ||
| - | + | Structural Basis for Regulation and Specificity of Fructooligosaccharide Import in Streptococcus pneumoniae.,Culurgioni S, Harris G, Singh AK, King SJ, Walsh MA Structure. 2017 Jan 3;25(1):79-93. doi: 10.1016/j.str.2016.11.008. Epub 2016 Dec , 8. PMID:27939783<ref>PMID:27939783</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Culurgioni | + | <div class="pdbe-citations 5g5z" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: King | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptococcus pneumoniae TIGR4]] | ||
| + | [[Category: Culurgioni S]] | ||
| + | [[Category: Harris G]] | ||
| + | [[Category: King SJ]] | ||
| + | [[Category: Singh AK]] | ||
| + | [[Category: Walsh MA]] | ||
Current revision
S.pneumoniae ABC-transporter substrate binding protein FusA in complex with kestose
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