5lfk

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'''Unreleased structure'''
 
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The entry 5lfk is ON HOLD
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==Crystal structure of CpxAHDC (hemiphosphorylated form)==
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<StructureSection load='5lfk' size='340' side='right'caption='[[5lfk]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5lfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LFK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.094&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NEP:N1-PHOSPHONOHISTIDINE'>NEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfk OCA], [https://pdbe.org/5lfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lfk RCSB], [https://www.ebi.ac.uk/pdbsum/5lfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPXA_ECOLI CPXA_ECOLI] This protein is involved in several diverse cellular processes, such as the functioning of acetohydroxyacid synthetase I, in the biosynthesis of isoleucine and valine, the TraJ protein activation activity for tra gene expression in F plasmid, and the synthesis, translocation, or stability of cell envelope proteins. Activates CpxR by phosphorylation.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial two-component systems consist of a sensor histidine kinase (HK) and a response regulator (RR). HKs are homodimers that catalyze the autophosphorylation of a histidine residue and the subsequent phosphoryl transfer to its RR partner, triggering an adaptive response. How the HK autokinase and phosphotransferase activities are coordinated remains unclear. Here, we report X-ray structures of the prototypical HK CpxA trapped as a hemi-phosphorylated dimer, and of the receiver domain from the RR partner, CpxR. Our results reveal that the two catalytic reactions can occur simultaneously, one in each protomer of the asymmetric CpxA dimer. Furthermore, the increase of autokinase activity in the presence of phosphotransfer-impaired CpxR put forward the idea of an allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer. The ensuing dynamical model provides a mechanistic explanation of how HKs can efficiently orchestrate two catalytic reactions involving large-scale protein motions.
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Authors: Mechaly, A.E., Alzari, P.M.
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Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase.,Mechaly AE, Soto Diaz S, Sassoon N, Buschiazzo A, Betton JM, Alzari PM Structure. 2017 Jun 6;25(6):939-944.e3. doi: 10.1016/j.str.2017.04.011. Epub 2017, May 25. PMID:28552574<ref>PMID:28552574</ref>
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Description: Crystal structure of CpxAHDC (hemiphosphorylated form)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Alzari, P.M]]
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<div class="pdbe-citations 5lfk" style="background-color:#fffaf0;"></div>
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[[Category: Mechaly, A.E]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Alzari PM]]
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[[Category: Mechaly AE]]

Current revision

Crystal structure of CpxAHDC (hemiphosphorylated form)

PDB ID 5lfk

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