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1a0b

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[[Image:1a0b.gif|left|200px]]<br />
 
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<applet load="1a0b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a0b, resolution 2.06&Aring;" />
 
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'''HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI'''<br />
 
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==Overview==
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==HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI==
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The histidine-containing phosphotransfer (HPt) domain is a novel protein, module with an active histidine residue that mediates phosphotransfer, reactions in the two-component signaling systems. A multistep phosphorelay, involving the HPt domain has been suggested for these signaling pathways., The crystal structure of the HPt domain of the anaerobic sensor kinase, ArcB has been determined at 2.06 A resolution. The domain consists of six, alpha helices containing a four-helix bundle-folding. The pattern of, sequence similarity of the HPt domains of ArcB and components in other, signaling systems can be interpreted in light of the three-dimensional, structure and supports the conclusion that the HPt domains have a common, structural motif both in prokaryotes and eukaryotes.
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<StructureSection load='1a0b' size='340' side='right'caption='[[1a0b]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1a0b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A0B FirstGlance]. <br>
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1A0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Sites: PHB and ZNB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A0B OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a0b OCA], [https://pdbe.org/1a0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a0b RCSB], [https://www.ebi.ac.uk/pdbsum/1a0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a0b ProSAT]</span></td></tr>
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Insights into multistep phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB., Kato M, Mizuno T, Shimizu T, Hakoshima T, Cell. 1997 Mar 7;88(5):717-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9054511 9054511]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARCB_ECOLI ARCB_ECOLI] Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/1a0b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a0b ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hakoshima, T.]]
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[[Category: Hakoshima T]]
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[[Category: Kato, M.]]
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[[Category: Kato M]]
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[[Category: Mizuno, T.]]
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[[Category: Mizuno T]]
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[[Category: Shimizu, T.]]
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[[Category: Shimizu T]]
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[[Category: ZN]]
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[[Category: four-helix bundle]]
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[[Category: histidine kinase]]
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[[Category: phosphotransfer]]
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[[Category: two-component system]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:43:15 2007''
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Current revision

HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI

PDB ID 1a0b

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