1jw9

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[[Image:1jw9.gif|left|200px]]
 
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{{Structure
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==Structure of the Native MoeB-MoaD Protein Complex==
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|PDB= 1jw9 |SIZE=350|CAPTION= <scene name='initialview01'>1jw9</scene>, resolution 1.7&Aring;
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<StructureSection load='1jw9' size='340' side='right'caption='[[1jw9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1jw9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JW9 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= MoeB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), MoaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jw9 OCA], [https://pdbe.org/1jw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jw9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jw9 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1fmo|1FMO]], [[1jwa|1JWA]], [[1jwb|1JWB]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jw9 OCA], [http://www.ebi.ac.uk/pdbsum/1jw9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jw9 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MOEB_ECOLI MOEB_ECOLI] Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein MoaD.<ref>PMID:11290749</ref> <ref>PMID:11463785</ref>
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== Evolutionary Conservation ==
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'''Structure of the Native MoeB-MoaD Protein Complex'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jw9_consurf.spt"</scriptWhenChecked>
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The activation of ubiquitin and related protein modifiers is catalysed by members of the E1 enzyme family that use ATP for the covalent self-attachment of the modifiers to a conserved cysteine. The Escherichia coli proteins MoeB and MoaD are involved in molybdenum cofactor (Moco) biosynthesis, an evolutionarily conserved pathway. The MoeB- and E1-catalysed reactions are mechanistically similar, and despite a lack of sequence similarity, MoaD and ubiquitin display the same fold including a conserved carboxy-terminal Gly-Gly motif. Similar to the E1 enzymes, MoeB activates the C terminus of MoaD to form an acyl-adenylate. Subsequently, a sulphurtransferase converts the MoaD acyl-adenylate to a thiocarboxylate that acts as the sulphur donor during Moco biosynthesis. These findings suggest that ubiquitin and E1 are derived from two ancestral genes closely related to moaD and moeB. Here we present the crystal structures of the MoeB-MoaD complex in its apo, ATP-bound, and MoaD-adenylate forms, and highlight the functional similarities between the MoeB- and E1-substrate complexes. These structures provide a molecular framework for understanding the activation of ubiquitin, Rub, SUMO and the sulphur incorporation step during Moco and thiamine biosynthesis.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1JW9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JW9 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jw9 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex., Lake MW, Wuebbens MM, Rajagopalan KV, Schindelin H, Nature. 2001 Nov 15;414(6861):325-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11713534 11713534]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Lake, M W.]]
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[[Category: Lake MW]]
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[[Category: Rajagopalan, K V.]]
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[[Category: Rajagopalan KV]]
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[[Category: Schindelin, H.]]
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[[Category: Schindelin H]]
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[[Category: Wuebbens, M M.]]
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[[Category: Wuebbens MM]]
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[[Category: (2) cys-x-x-cys zinc-binding motif]]
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[[Category: moad: ubiquitin-like fold]]
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[[Category: moeb: modified rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:53 2008''
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Current revision

Structure of the Native MoeB-MoaD Protein Complex

PDB ID 1jw9

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OCA

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