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1jwf

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[[Image:1jwf.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of human GGA1 VHS domain.==
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|PDB= 1jwf |SIZE=350|CAPTION= <scene name='initialview01'>1jwf</scene>, resolution 2.100&Aring;
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<StructureSection load='1jwf' size='340' side='right'caption='[[1jwf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1jwf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWF FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwf OCA], [https://pdbe.org/1jwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwf RCSB], [https://www.ebi.ac.uk/pdbsum/1jwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwf ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1jwg|1JWG]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwf OCA], [http://www.ebi.ac.uk/pdbsum/1jwf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jwf RCSB]</span>
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[https://www.uniprot.org/uniprot/GGA1_HUMAN GGA1_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Crystal Structure of human GGA1 VHS domain.'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwf_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-interacting proteins) are critical for the transport of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ADP-ribosylation factor (ARF), and clathrin. The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster dileucine (ACLL) sequences. Here we report the X-ray structure of the GGA1 VHS domain alone, and in complex with the carboxy-terminal peptide of cation-independent mannose 6-phosphate receptor containing an ACLL sequence. The VHS domain forms a super helix with eight alpha-helices, similar to the VHS domains of TOM1 and Hrs. Unidirectional movements of helices alpha6 and alpha8, and some of their side chains, create a set of electrostatic and hydrophobic interactions for correct recognition of the ACLL peptide. This recognition mechanism provides the basis for regulation of protein transport from the TGN to endosomes/lysosomes, which is shared by sortilin and low-density lipoprotein receptor-related protein.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwf ConSurf].
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1JWF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWF OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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Structural basis for recognition of acidic-cluster dileucine sequence by GGA1., Shiba T, Takatsu H, Nogi T, Matsugaki N, Kawasaki M, Igarashi N, Suzuki M, Kato R, Earnest T, Nakayama K, Wakatsuki S, Nature. 2002 Feb 21;415(6874):937-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11859376 11859376]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Earnest, T.]]
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[[Category: Earnest T]]
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[[Category: Igarashi, N.]]
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[[Category: Igarashi N]]
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[[Category: Kato, R.]]
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[[Category: Kato R]]
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[[Category: Kawasaki, M.]]
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[[Category: Kawasaki M]]
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[[Category: Matsugaki, N]]
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[[Category: Matsugaki N]]
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[[Category: Nakayama, K.]]
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[[Category: Nakayama K]]
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[[Category: Nogi, T.]]
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[[Category: Nogi T]]
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[[Category: Shiba, T.]]
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[[Category: Shiba T]]
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[[Category: Suzuki, M.]]
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[[Category: Suzuki M]]
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[[Category: Takatsu, H.]]
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[[Category: Takatsu H]]
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[[Category: Wakatsuki, S.]]
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[[Category: Wakatsuki S]]
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[[Category: super helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:58 2008''
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Current revision

Crystal Structure of human GGA1 VHS domain.

PDB ID 1jwf

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