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5eeg

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==Crystal structure of carminomycin-4-O-methyltransferase DnrK in complex with tetrazole-SAH==
==Crystal structure of carminomycin-4-O-methyltransferase DnrK in complex with tetrazole-SAH==
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<StructureSection load='5eeg' size='340' side='right' caption='[[5eeg]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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<StructureSection load='5eeg' size='340' side='right'caption='[[5eeg]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5eeg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EEG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5eeg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EEG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=S8M:(2~{R},3~{R},4~{S},5~{S})-2-(6-AMINOPURIN-9-YL)-5-[[(3~{S})-3-AZANYL-3-(1~{H}-1,2,3,4-TETRAZOL-5-YL)PROPYL]SULFANYLMETHYL]OXOLANE-3,4-DIOL'>S8M</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.255&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eeh|5eeh]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=S8M:(2~{R},3~{R},4~{S},5~{S})-2-(6-AMINOPURIN-9-YL)-5-[[(3~{S})-3-AZANYL-3-(1~{H}-1,2,3,4-TETRAZOL-5-YL)PROPYL]SULFANYLMETHYL]OXOLANE-3,4-DIOL'>S8M</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carminomycin_4-O-methyltransferase Carminomycin 4-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.292 2.1.1.292] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eeg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eeg OCA], [https://pdbe.org/5eeg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eeg RCSB], [https://www.ebi.ac.uk/pdbsum/5eeg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eeg ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eeg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eeg OCA], [http://pdbe.org/5eeg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eeg RCSB], [http://www.ebi.ac.uk/pdbsum/5eeg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eeg ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/DNRK_STRPE DNRK_STRPE]] Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.<ref>PMID:15273252</ref>
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[https://www.uniprot.org/uniprot/DNRK_STRPE DNRK_STRPE] Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.<ref>PMID:15273252</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Carminomycin 4-O-methyltransferase]]
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[[Category: Large Structures]]
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[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
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[[Category: Streptomyces peucetius]]
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[[Category: Phillips, G N]]
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[[Category: Phillips Jr GN]]
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[[Category: Singh, S]]
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[[Category: Singh S]]
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[[Category: Thorson, J S]]
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[[Category: Thorson JS]]
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[[Category: Wang, F]]
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[[Category: Wang F]]
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[[Category: Enzyme discovery for natural product biosynthesis]]
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[[Category: Natpro]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Psi-biology]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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[[Category: Unnatural substrate]]
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Current revision

Crystal structure of carminomycin-4-O-methyltransferase DnrK in complex with tetrazole-SAH

PDB ID 5eeg

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