5l5m

From Proteopedia

(Difference between revisions)

Current revision

Plexin A4 full extracellular region, domains 1 to 7 modeled, data to 8 angstrom, spacegroup P4(3)2(1)2

Structural highlights

5l5m is a 1 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLXA4_MOUSE Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.^[1] ^[2]

Publication Abstract from PubMed

Class A plexins (PlxnAs) act as semaphorin receptors and control diverse aspects of nervous system development and plasticity, ranging from axon guidance and neuron migration to synaptic organization. PlxnA signaling requires cytoplasmic domain dimerization, but extracellular regulation and activation mechanisms remain unclear. Here we present crystal structures of PlxnA (PlxnA1, PlxnA2, and PlxnA4) full ectodomains. Domains 1-9 form a ring-like conformation from which the C-terminal domain 10 points away. All our PlxnA ectodomain structures show autoinhibitory, intermolecular "head-to-stalk" (domain 1 to domain 4-5) interactions, which are confirmed by biophysical assays, live cell fluorescence microscopy, and cell-based and neuronal growth cone collapse assays. This work reveals a 2-fold role of the PlxnA ectodomains: imposing a pre-signaling autoinhibitory separation for the cytoplasmic domains via intermolecular head-to-stalk interactions and supporting dimerization-based PlxnA activation upon ligand binding. More generally, our data identify a novel molecular mechanism for preventing premature activation of axon guidance receptors.

Structural Basis for Plexin Activation and Regulation.,Kong Y, Janssen BJ, Malinauskas T, Vangoor VR, Coles CH, Kaufmann R, Ni T, Gilbert RJ, Padilla-Parra S, Pasterkamp RJ, Jones EY Neuron. 2016 Jul 5. pii: S0896-6273(16)30295-1. doi:, 10.1016/j.neuron.2016.06.018. PMID:27397516^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Suto F, Murakami Y, Nakamura F, Goshima Y, Fujisawa H. Identification and characterization of a novel mouse plexin, plexin-A4. Mech Dev. 2003 Mar;120(3):385-96. PMID:12591607
↑ Waimey KE, Huang PH, Chen M, Cheng HJ. Plexin-A3 and plexin-A4 restrict the migration of sympathetic neurons but not their neural crest precursors. Dev Biol. 2008 Mar 15;315(2):448-58. doi: 10.1016/j.ydbio.2008.01.002. Epub 2008 , Jan 16. PMID:18262512 doi:http://dx.doi.org/10.1016/j.ydbio.2008.01.002
↑ Kong Y, Janssen BJ, Malinauskas T, Vangoor VR, Coles CH, Kaufmann R, Ni T, Gilbert RJ, Padilla-Parra S, Pasterkamp RJ, Jones EY. Structural Basis for Plexin Activation and Regulation. Neuron. 2016 Jul 5. pii: S0896-6273(16)30295-1. doi:, 10.1016/j.neuron.2016.06.018. PMID:27397516 doi:http://dx.doi.org/10.1016/j.neuron.2016.06.018

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5l5m"

@@ Line 1: / Line 1: @@
 ==Plexin A4 full extracellular region, domains 1 to 7 modeled, data to 8 angstrom, spacegroup P4(3)2(1)2==
-<StructureSection load='5l5m' size='340' side='right' caption='[[5l5m]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
+<StructureSection load='5l5m' size='340' side='right'caption='[[5l5m]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5l5m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L5M FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5l5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L5M FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l5m OCA], [http://pdbe.org/5l5m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l5m RCSB], [http://www.ebi.ac.uk/pdbsum/5l5m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l5m ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l5m OCA], [https://pdbe.org/5l5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l5m RCSB], [https://www.ebi.ac.uk/pdbsum/5l5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l5m ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PLXA4_MOUSE PLXA4_MOUSE]] Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.<ref>PMID:12591607</ref> <ref>PMID:18262512</ref>
+[https://www.uniprot.org/uniprot/PLXA4_MOUSE PLXA4_MOUSE] Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.<ref>PMID:12591607</ref> <ref>PMID:18262512</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 </div>
 <div class="pdbe-citations 5l5m" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Plexin 3D structures|Plexin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Coles, C H]]
+[[Category: Large Structures]]
-[[Category: Gilbert, R J.C]]
+[[Category: Mus musculus]]
-[[Category: Janssen, B J.C]]
+[[Category: Coles CH]]
-[[Category: Jones, E Y]]
+[[Category: Gilbert RJC]]
-[[Category: Kaufmann, R]]
+[[Category: Janssen BJC]]
-[[Category: Kong, Y]]
+[[Category: Jones EY]]
-[[Category: Malinauskas, T]]
+[[Category: Kaufmann R]]
-[[Category: Ni, T]]
+[[Category: Kong Y]]
-[[Category: Padilla-Parra, S]]
+[[Category: Malinauskas T]]
-[[Category: Pasterkamp, R J]]
+[[Category: Ni T]]
-[[Category: Vangoor, V R]]
+[[Category: Padilla-Parra S]]
-[[Category: Axon guidance]]
+[[Category: Pasterkamp RJ]]
-[[Category: Receptor]]
+[[Category: Vangoor VR]]
-[[Category: Signaling]]
-[[Category: Signaling protein]]

5l5m

From Proteopedia

Current revision

Plexin A4 full extracellular region, domains 1 to 7 modeled, data to 8 angstrom, spacegroup P4(3)2(1)2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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