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| | ==Structure of a complex between S. cerevisiae Csm1 and Mam1== | | ==Structure of a complex between S. cerevisiae Csm1 and Mam1== |
| - | <StructureSection load='5ktb' size='340' side='right' caption='[[5ktb]], [[Resolution|resolution]] 3.05Å' scene=''> | + | <StructureSection load='5ktb' size='340' side='right'caption='[[5ktb]], [[Resolution|resolution]] 3.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ktb]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4emc 4emc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KTB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ktb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4emc 4emc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KTB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ktb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ktb OCA], [http://pdbe.org/5ktb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ktb RCSB], [http://www.ebi.ac.uk/pdbsum/5ktb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ktb ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ktb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ktb OCA], [https://pdbe.org/5ktb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ktb RCSB], [https://www.ebi.ac.uk/pdbsum/5ktb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ktb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CSM1_YEAST CSM1_YEAST]] Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Plays also a mitotic role in DNA replication.<ref>PMID:11470404</ref> <ref>PMID:12689592</ref> <ref>PMID:15023545</ref> <ref>PMID:16487579</ref> <ref>PMID:18997772</ref> [[http://www.uniprot.org/uniprot/MAM1_YEAST MAM1_YEAST]] Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis.<ref>PMID:11163190</ref> <ref>PMID:11470404</ref> <ref>PMID:12689592</ref> <ref>PMID:12586695</ref> <ref>PMID:12717442</ref> <ref>PMID:12663816</ref> <ref>PMID:15620645</ref> <ref>PMID:15226378</ref> | + | [https://www.uniprot.org/uniprot/CSM1_YEAST CSM1_YEAST] Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Plays also a mitotic role in DNA replication.<ref>PMID:11470404</ref> <ref>PMID:12689592</ref> <ref>PMID:15023545</ref> <ref>PMID:16487579</ref> <ref>PMID:18997772</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Corbett, K D]] | + | [[Category: Large Structures]] |
| - | [[Category: Harrison, S C]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Monopolin]] | + | [[Category: Corbett KD]] |
| - | [[Category: Replication]] | + | [[Category: Harrison SC]] |
| Structural highlights
Function
CSM1_YEAST Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Plays also a mitotic role in DNA replication.[1] [2] [3] [4] [5]
Publication Abstract from PubMed
The Saccharomyces cerevisiae monopolin complex directs proper chromosome segregation in meiosis I by mediating co-orientation of sister kinetochores on the meiosis I spindle. The monopolin subunits Csm1 and Lrs4 form a V-shaped complex that may directly crosslink sister kinetochores. We report here biochemical characterization of the monopolin complex subunits Mam1 and Hrr25 and of the complete four-protein monopolin complex. By purifying monopolin subcomplexes with different subunit combinations, we have determined the stoichiometry and overall architecture of the full monopolin complex. We have determined the crystal structure of Csm1 bound to a Mam1 fragment, showing how Mam1 wraps around the Csm1 dimer and alters the stoichiometry of kinetochore-protein binding by Csm1. We further show that the kinase activity of Hrr25 is altered by Mam1 binding, and we identify Hrr25 phosphorylation sites on Mam1 that may affect monopolin complex stability and/or kinetochore binding in meiosis.
Molecular architecture of the yeast monopolin complex.,Corbett KD, Harrison SC Cell Rep. 2012 Jun 28;1(6):583-9. Epub 2012 Jun 21. PMID:22813733[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rabitsch KP, Toth A, Galova M, Schleiffer A, Schaffner G, Aigner E, Rupp C, Penkner AM, Moreno-Borchart AC, Primig M, Esposito RE, Klein F, Knop M, Nasmyth K. A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol. 2001 Jul 10;11(13):1001-9. PMID:11470404
- ↑ Rabitsch KP, Petronczki M, Javerzat JP, Genier S, Chwalla B, Schleiffer A, Tanaka TU, Nasmyth K. Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I. Dev Cell. 2003 Apr;4(4):535-48. PMID:12689592
- ↑ Wysocka M, Rytka J, Kurlandzka A. Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex. Exp Cell Res. 2004 Apr 1;294(2):592-602. PMID:15023545 doi:10.1016/j.yexcr.2003.12.008
- ↑ Pan X, Ye P, Yuan DS, Wang X, Bader JS, Boeke JD. A DNA integrity network in the yeast Saccharomyces cerevisiae. Cell. 2006 Mar 10;124(5):1069-81. Epub 2006 Feb 16. PMID:16487579 doi:S0092-8674(06)00118-8
- ↑ Mekhail K, Seebacher J, Gygi SP, Moazed D. Role for perinuclear chromosome tethering in maintenance of genome stability. Nature. 2008 Dec 4;456(7222):667-70. doi: 10.1038/nature07460. Epub 2008 Nov 9. PMID:18997772 doi:10.1038/nature07460
- ↑ Corbett KD, Harrison SC. Molecular architecture of the yeast monopolin complex. Cell Rep. 2012 Jun 28;1(6):583-9. Epub 2012 Jun 21. PMID:22813733 doi:10.1016/j.celrep.2012.05.012
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