1k6g

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[[Image:1k6g.gif|left|200px]]
 
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{{Structure
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==Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing==
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|PDB= 1k6g |SIZE=350|CAPTION= <scene name='initialview01'>1k6g</scene>
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<StructureSection load='1k6g' size='340' side='right'caption='[[1k6g]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[1k6g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K6G FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6g OCA], [https://pdbe.org/1k6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k6g RCSB], [https://www.ebi.ac.uk/pdbsum/1k6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k6g ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1k6h|1K6H]]
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<div style="background-color:#fffaf0;">
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6g OCA], [http://www.ebi.ac.uk/pdbsum/1k6g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k6g RCSB]</span>
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== Publication Abstract from PubMed ==
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}}
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'''Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing'''
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==Overview==
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Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage.
Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage.
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==About this Structure==
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Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing.,Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D EMBO J. 2001 Dec 17;20(24):7250-8. PMID:11743001<ref>PMID:11743001</ref>
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1K6G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6G OCA].
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==Reference==
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Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743001 11743001]
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[[Category: Protein complex]]
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[[Category: Elela, S Abou.]]
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[[Category: Fourmy, D.]]
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[[Category: Lamontagne, B.]]
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[[Category: Lebars, I.]]
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[[Category: Yoshizawa, S.]]
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[[Category: rna tetraloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:44:06 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1k6g" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Abou Elela S]]
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[[Category: Fourmy D]]
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[[Category: Lamontagne B]]
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[[Category: Lebars I]]
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[[Category: Yoshizawa S]]

Current revision

Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing

PDB ID 1k6g

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