5gn8

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'''Unreleased structure'''
 
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The entry 5gn8 is ON HOLD
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==Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign==
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<StructureSection load='5gn8' size='340' side='right'caption='[[5gn8]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5gn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GN8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.805&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gn8 OCA], [https://pdbe.org/5gn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gn8 RCSB], [https://www.ebi.ac.uk/pdbsum/5gn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gn8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FRIH_HUMAN FRIH_HUMAN] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Rendering the geometry of protein-based assemblies controllable remains challenging. Protein shell-like nanocages represent particularly interesting targets for designed assembly. Here, we introduce an engineering strategy-key subunit interface redesign (KSIR)-that alters a natural subunit-subunit interface by selective deletion of a small number of "silent" amino acid residues (no participation in interfacial interactions) into one that triggers the generation of a non-native protein cage. We have applied KSIR to construct a non-native 48-mer nanocage from its native 24-mer recombinant human H-chain ferritin (rHuHF). This protein is a heteropolymer composed of equal numbers of two different subunits which are derived from one polypeptide. This strategy has allowed the study of conversion between protein nanocages with different geometries by re-engineering key subunit interfaces and the demonstration of the important role of the above-mentioned specific residues in providing geometric specificity for protein assembly.
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Authors: Zhang, S., Zang, J., Zhao, G., Mikami, B.
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"Silent" Amino Acid Residues at Key Subunit Interfaces Regulate the Geometry of Protein Nanocages.,Zhang S, Zang J, Zhang X, Chen H, Mikami B, Zhao G ACS Nano. 2016 Nov 22;10(11):10382-10388. Epub 2016 Nov 9. PMID:27934076<ref>PMID:27934076</ref>
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Description: Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Zhang, S]]
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<div class="pdbe-citations 5gn8" style="background-color:#fffaf0;"></div>
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[[Category: Mikami, B]]
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[[Category: Zang, J]]
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==See Also==
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[[Category: Zhao, G]]
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Mikami B]]
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[[Category: Zang J]]
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[[Category: Zhang S]]
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[[Category: Zhao G]]

Current revision

Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

PDB ID 5gn8

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