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| - | [[Image:1k9g.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal Structure of the Complex of Cryptolepine-d(CCTAGG)2== |
| - | |PDB= 1k9g |SIZE=350|CAPTION= <scene name='initialview01'>1k9g</scene>, resolution 1.4Å
| + | <StructureSection load='1k9g' size='340' side='right'caption='[[1k9g]], [[Resolution|resolution]] 1.40Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DR1:5-METHYL-5H-INDOLO[3,2-B]QUINOLINE'>DR1</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1k9g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K9G FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DR1:5-METHYL-5H-INDOLO[3,2-B]QUINOLINE'>DR1</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k9g OCA], [https://pdbe.org/1k9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k9g RCSB], [https://www.ebi.ac.uk/pdbsum/1k9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9g ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k9g OCA], [http://www.ebi.ac.uk/pdbsum/1k9g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k9g RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Crystal Structure of the Complex of Cryptolepine-d(CCTAGG)2'''
| + | [[Category: Aymami J]] |
| - | | + | [[Category: Coll M]] |
| - | | + | [[Category: Lisgarten JN]] |
| - | ==Overview==
| + | [[Category: Portugal J]] |
| - | Cryptolepine, a naturally occurring indoloquinoline alkaloid used as an antimalarial drug in Central and Western Africa, has been found to bind to DNA in a formerly unknown intercalation mode. Evidence from competition dialysis assays demonstrates that cryptolepine is able to bind CG-rich sequences containing nonalternating CC sites. Here we show that cryptolepine interacts with the CC sites of the DNA fragment d(CCTAGG)(2) in a base-stacking intercalation mode. This is the first DNA intercalator complex, from approximately 90 solved by X-ray crystallography, to bind a nonalternating (pyrimidine-pyrimidine) DNA sequence. The asymmetry of the drug induces a perfect stacking with the asymmetric site, allowing for the stability of the complex in the absence of hydrogen bonding interactions. The crystal structure of this antimalarial drug-DNA complex provides evidence for the first nonalternating intercalation and, as such, provides a basis for the design of new anticancer or antimalarial drugs.
| + | [[Category: Wright CW]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1K9G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9G OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | The antimalarial and cytotoxic drug cryptolepine intercalates into DNA at cytosine-cytosine sites., Lisgarten JN, Coll M, Portugal J, Wright CW, Aymami J, Nat Struct Biol. 2002 Jan;9(1):57-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11731803 11731803]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Aymami, J.]] | + | |
| - | [[Category: Coll, M.]] | + | |
| - | [[Category: Lisgarten, J N.]] | + | |
| - | [[Category: Portugal, J.]] | + | |
| - | [[Category: Wright, C W.]] | + | |
| - | [[Category: dna intercalator complex]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:45:17 2008''
| + | |
