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| | ==Structure of strictosidine synthase in complex with 2-(1H-INDOL-3-YL)-N-METHYLETHANAMINE== | | ==Structure of strictosidine synthase in complex with 2-(1H-INDOL-3-YL)-N-METHYLETHANAMINE== |
| - | <StructureSection load='4iyg' size='340' side='right' caption='[[4iyg]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='4iyg' size='340' side='right'caption='[[4iyg]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4iyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Devilpepper Devilpepper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IYG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4iyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IYG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1HU:2-(1H-INDOL-3-YL)-N-METHYLETHANAMINE'>1HU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.702Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4060 Devilpepper])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1HU:2-(1H-INDOL-3-YL)-N-METHYLETHANAMINE'>1HU</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iyg OCA], [https://pdbe.org/4iyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iyg RCSB], [https://www.ebi.ac.uk/pdbsum/4iyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iyg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iyg OCA], [http://pdbe.org/4iyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iyg RCSB], [http://www.ebi.ac.uk/pdbsum/4iyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iyg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE]] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis. | + | [https://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Devilpepper]] | + | [[Category: Large Structures]] |
| - | [[Category: Strictosidine synthase]] | + | [[Category: Rauvolfia serpentina]] |
| - | [[Category: Fangrui, W]] | + | [[Category: Fangrui W]] |
| - | [[Category: Rajendran, C]] | + | [[Category: Rajendran C]] |
| - | [[Category: Stoeckigt, J]] | + | [[Category: Stoeckigt J]] |
| - | [[Category: Wang, M]] | + | [[Category: Wang M]] |
| - | [[Category: Lyase-lyase inhibitor complex]]
| + | |
| Structural highlights
Function
STSY_RAUSE Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
Publication Abstract from PubMed
The Pictet-Spenglerasestrictosidine synthase (STR) has been characterized as the central enzyme in the biosynthesis of around 2000 monoterpenoid indole alkaloids in plants. In the light of a high therapeutic value and huge scaffold diversity these alkaloids represent, STR as an enzyme has attracted great attentions in recent years, intending to be utilized in the formation of new interesting alkaloids with unusual substitution pattern or even with novel scaffolds. For outlining the application potential that STR possesses, together with insight into the reaction mechanism catalyzed by STR, strategies and methods for exploring the applicability of STR have been updated in this article by taking R. serpertina STR(RS-STR) and C. roseus.STR (CR-STR) as representative models, followed by introducing the latest released complex structures of RS-STR with new substrates. Examples provided here, including substrate scaffold tailoring, X-ray crystal complex structure comparison, protein engineering and biosynthetic pathway reprogramming, pave the way to finally construct novel alkaloids libraries by chemo-enzymatic approaches.
Using Strictosidine Synthase to Prepare Novel Alkaloids.,Zhu H, Kercmar P, Wu F, Rajendran C, Sun L, Wang M, Stockigt J Curr Med Chem. 2015 Apr 8. PMID:25850764[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhu H, Kercmar P, Wu F, Rajendran C, Sun L, Wang M, Stockigt J. Using Strictosidine Synthase to Prepare Novel Alkaloids. Curr Med Chem. 2015 Apr 8. PMID:25850764
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