5ghe

<StructureSection load='5ghe' size='340' side='right' caption='[[5ghe]], [[Resolution|resolution]] 1.90&Aring;' scene=''>

<table><tr><td colspan='2'>[[5ghe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GHE FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ghe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ghe OCA], [http://pdbe.org/5ghe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ghe RCSB], [http://www.ebi.ac.uk/pdbsum/5ghe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ghe ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/CR6AA_BACTU CR6AA_BACTU]] Endotoxin with nematicidal activity.

Crystal (Cry) proteins from Bacillus thuringiensis (Bt) are globally used in agriculture as proteinaceous insecticides. Numerous crystal structures have been determined, and most exhibit conserved three-dimensional architectures. Recently, we have identified a novel nematicidal mechanism by which Cry6Aa triggers cell death through a necrosis-signaling pathway via an interaction with the host protease ASP-1. However, we found little sequence conservation of Cry6Aa in our functional study. Here, we report the 1.90 angstrom (A) resolution structure of the proteolytic form of Cry6Aa (1-396), determined by X-ray crystallography. The structure of Cry6Aa is highly similar to those of the pathogenic toxin family of ClyA-type alpha-pore-forming toxins (alpha-PFTs), which are characterized by a bipartite structure comprising a head domain and a tail domain, thus suggesting that Cry6Aa exhibits a previously undescribed nematicidal mode of action. This structure also provides a framework for the functional study of other nematicidal toxins.

 

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== References ==

[[Category: Guan, Z]]

[[Category: Huang, J]]

[[Category: Sun, M]]

[[Category: Yin, P]]

[[Category: Zou, T]]

[[Category: Clya-type]]

[[Category: Cry6aa2]]

[[Category: Toxin]]