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| - | ==Crystal Strucutre of UDP-glucose Pyrophosphorylase of Homo Sapiens== | + | ==Crystal Structure of UDP-glucose Pyrophosphorylase of Homo Sapiens== |
| - | <StructureSection load='3r2w' size='340' side='right' caption='[[3r2w]], [[Resolution|resolution]] 3.60Å' scene=''> | + | <StructureSection load='3r2w' size='340' side='right'caption='[[3r2w]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3r2w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3r2w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2W FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UGP2, UGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UTP--glucose-1-phosphate_uridylyltransferase UTP--glucose-1-phosphate uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.9 2.7.7.9] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2w OCA], [https://pdbe.org/3r2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2w RCSB], [https://www.ebi.ac.uk/pdbsum/3r2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2w ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2w OCA], [http://pdbe.org/3r2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r2w RCSB], [http://www.ebi.ac.uk/pdbsum/3r2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UGPA_HUMAN UGPA_HUMAN]] Plays a central role as a glucosyl donor in cellular metabolic pathways. | + | [https://www.uniprot.org/uniprot/UGPA_HUMAN UGPA_HUMAN] Plays a central role as a glucosyl donor in cellular metabolic pathways. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
| + | |
| - | UDP-glucose pyrophosphorylase (UGPase) is highly conserved among eukaryotes. UGPase reversely catalyzes the formation of UDP-glucose and is critical in carbohydrate metabolism. Previous studies have mainly focused on the UGPases from plants, fungi and parasites and indicate that the regulatory mechanisms responsible for the enzyme activity vary among different organisms. Here the crystal structure of human UGPase (hUGPase) was determined and shown to form octamers through end-to-end and side-by-side interactions. The observed latch loop in hUGPase distinctly differs from yeast UGPase (yUGPase), which could explain why hUGPase and yUGPase possess different enzymatic activities. Mutagenesis studies showed that both dissociation of octamers and mutations of the latch loop can significantly affect the UGPase activity. Moreover, this latch effect is also evolutionarily meaningful in UGPase from different species.
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| - | | + | |
| - | The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect that influence enzymatic activity.,Yu Q, Zheng X Biochem J. 2011 Dec 1. PMID:22132858<ref>PMID:22132858</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3r2w" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: UTP--glucose-1-phosphate uridylyltransferase]] | + | [[Category: Large Structures]] |
| - | [[Category: Yu, Q]] | + | [[Category: Yu Q]] |
| - | [[Category: Zheng, X]] | + | [[Category: Zheng X]] |
| - | [[Category: Homo sapien]]
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| - | [[Category: Nucleotidyltransferase]]
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| - | [[Category: Rossmann fold beta barrel]]
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| - | [[Category: Transferase]]
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