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| | ==Crystal Structure of Ad37 fiber knob in complex with GD1a oligosaccharide== | | ==Crystal Structure of Ad37 fiber knob in complex with GD1a oligosaccharide== |
| - | <StructureSection load='3n0i' size='340' side='right' caption='[[3n0i]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='3n0i' size='340' side='right'caption='[[3n0i]], [[Resolution|resolution]] 1.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3n0i]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Adenovirus_type_37 Adenovirus type 37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N0I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3n0i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_D37 Human adenovirus D37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N0I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=52275 Adenovirus type 37])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0i OCA], [https://pdbe.org/3n0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n0i RCSB], [https://www.ebi.ac.uk/pdbsum/3n0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0i ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0i OCA], [http://pdbe.org/3n0i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n0i RCSB], [http://www.ebi.ac.uk/pdbsum/3n0i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0i ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q64823_9ADEN Q64823_9ADEN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Adenovirus type 37]] | + | [[Category: Human adenovirus D37]] |
| - | [[Category: Angstroem, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Arnberg, N]] | + | [[Category: Angstroem J]] |
| - | [[Category: Bauer, J]] | + | [[Category: Arnberg N]] |
| - | [[Category: Domelloef, F P]] | + | [[Category: Bauer J]] |
| - | [[Category: Fraengsmyr, L]] | + | [[Category: Domelloef FP]] |
| - | [[Category: Hedenstroem, M]] | + | [[Category: Fraengsmyr L]] |
| - | [[Category: Johansson, S M.C]] | + | [[Category: Hedenstroem M]] |
| - | [[Category: Lookene, A]] | + | [[Category: Johansson SMC]] |
| - | [[Category: Nilsson, E C]] | + | [[Category: Lookene A]] |
| - | [[Category: Rinaldi, S]] | + | [[Category: Nilsson EC]] |
| - | [[Category: Stehle, T]] | + | [[Category: Rinaldi S]] |
| - | [[Category: Storm, R J]] | + | [[Category: Stehle T]] |
| - | [[Category: Willison, H]] | + | [[Category: Storm RJ]] |
| - | [[Category: Adenovirus]]
| + | [[Category: Willison H]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Fiber knob]]
| + | |
| - | [[Category: Ganglioside]]
| + | |
| - | [[Category: Gd1a]]
| + | |
| - | [[Category: Oligosaccharide structure]]
| + | |
| - | [[Category: Protein carbohydrate interaction]]
| + | |
| - | [[Category: Virus-receptor complex]]
| + | |
| Structural highlights
3n0i is a 3 chain structure with sequence from Human adenovirus D37. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.65Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
Q64823_9ADEN
Publication Abstract from PubMed
Adenovirus type 37 (Ad37) is a leading cause of epidemic keratoconjunctivitis (EKC), a severe and highly contagious ocular disease. Whereas most other adenoviruses infect cells by engaging CD46 or the coxsackie and adenovirus receptor (CAR), Ad37 binds previously unknown sialic acid-containing cell surface molecules. By glycan array screening, we show here that the receptor-recognizing knob domain of the Ad37 fiber protein specifically binds a branched hexasaccharide that is present in the GD1a ganglioside and that features two terminal sialic acids. Soluble GD1a glycan and GD1a-binding antibodies efficiently prevented Ad37 virions from binding and infecting corneal cells. Unexpectedly, the receptor is constituted by one or more glycoproteins containing the GD1a glycan motif rather than the ganglioside itself, as shown by binding, infection and flow cytometry experiments. Molecular modeling, nuclear magnetic resonance and X-ray crystallography reveal that the two terminal sialic acids dock into two of three previously established sialic acid-binding sites in the trimeric Ad37 knob. Surface plasmon resonance analysis shows that the knob-GD1a glycan interaction has high affinity. Our findings therefore form a basis for the design and development of sialic acid-containing antiviral drugs for topical treatment of EKC.
The GD1a glycan is a cellular receptor for adenoviruses causing epidemic keratoconjunctivitis.,Nilsson EC, Storm RJ, Bauer J, Johansson SM, Lookene A, Angstrom J, Hedenstrom M, Eriksson TL, Frangsmyr L, Rinaldi S, Willison HJ, Domellof FP, Stehle T, Arnberg N Nat Med. 2010 Dec 12. PMID:21151139[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nilsson EC, Storm RJ, Bauer J, Johansson SM, Lookene A, Angstrom J, Hedenstrom M, Eriksson TL, Frangsmyr L, Rinaldi S, Willison HJ, Domellof FP, Stehle T, Arnberg N. The GD1a glycan is a cellular receptor for adenoviruses causing epidemic keratoconjunctivitis. Nat Med. 2010 Dec 12. PMID:21151139 doi:10.1038/nm.2267
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