4un5

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==Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3==
==Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3==
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<StructureSection load='4un5' size='340' side='right' caption='[[4un5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='4un5' size='340' side='right'caption='[[4un5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4un5]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UN5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4un5]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UN5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4un3|4un3]], [[4un4|4un4]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4un5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4un5 OCA], [http://pdbe.org/4un5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4un5 RCSB], [http://www.ebi.ac.uk/pdbsum/4un5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4un5 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4un5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4un5 OCA], [https://pdbe.org/4un5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4un5 RCSB], [https://www.ebi.ac.uk/pdbsum/4un5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4un5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
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[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
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*[[Anisotropic refinement|Anisotropic refinement]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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*[[Cas9|Cas9]]
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*[[Endonuclease|Endonuclease]]
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Anders, C]]
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[[Category: Large Structures]]
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[[Category: Duerst, A]]
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[[Category: Streptococcus pyogenes]]
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[[Category: Jinek, M]]
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[[Category: Synthetic construct]]
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[[Category: Niewoehner, O]]
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[[Category: Anders C]]
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[[Category: Genome editing]]
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[[Category: Duerst A]]
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[[Category: Hydrolase-dna-rna complex]]
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[[Category: Jinek M]]
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[[Category: Protein-rna complex]]
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[[Category: Niewoehner O]]
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[[Category: Rnp]]
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Current revision

Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3

PDB ID 4un5

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