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| | ==PB1 Domain of AtARF7 - SeMet Derivative== | | ==PB1 Domain of AtARF7 - SeMet Derivative== |
| - | <StructureSection load='4nj7' size='340' side='right' caption='[[4nj7]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='4nj7' size='340' side='right'caption='[[4nj7]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4nj7]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJ7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4nj7]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NJ7 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nj6|4nj6]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARF7, BIP, IAA21, IAA23, IAA25, NPH4, TIR5, At5g20730, T1M15.130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nj7 OCA], [https://pdbe.org/4nj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nj7 RCSB], [https://www.ebi.ac.uk/pdbsum/4nj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nj7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nj7 OCA], [http://pdbe.org/4nj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nj7 RCSB], [http://www.ebi.ac.uk/pdbsum/4nj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nj7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ARFG_ARATH ARFG_ARATH]] Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Required for differential growth responses of aerial tissues. Involved in ethylene responses. Regulates lateral root formation through direct regulation of LBD16 and/or LBD29. Functionally redundant with ARF19. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7.<ref>PMID:12036261</ref> <ref>PMID:14973283</ref> <ref>PMID:16461383</ref> <ref>PMID:17259263</ref> | + | [https://www.uniprot.org/uniprot/ARFG_ARATH ARFG_ARATH] Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Required for differential growth responses of aerial tissues. Involved in ethylene responses. Regulates lateral root formation through direct regulation of LBD16 and/or LBD29. Functionally redundant with ARF19. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7.<ref>PMID:12036261</ref> <ref>PMID:14973283</ref> <ref>PMID:16461383</ref> <ref>PMID:17259263</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Jez, J M]] | + | [[Category: Large Structures]] |
| - | [[Category: Korasick, D A]] | + | [[Category: Jez JM]] |
| - | [[Category: Lee, S G]] | + | [[Category: Korasick DA]] |
| - | [[Category: Nanao, M]] | + | [[Category: Lee SG]] |
| - | [[Category: Strader, L C]] | + | [[Category: Nanao M]] |
| - | [[Category: Westfall, C S]] | + | [[Category: Strader LC]] |
| - | [[Category: Beta-grasp fold]]
| + | [[Category: Westfall CS]] |
| - | [[Category: Pb1 domain]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
ARFG_ARATH Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Required for differential growth responses of aerial tissues. Involved in ethylene responses. Regulates lateral root formation through direct regulation of LBD16 and/or LBD29. Functionally redundant with ARF19. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7.[1] [2] [3] [4]
Publication Abstract from PubMed
In plants, the AUXIN RESPONSE FACTOR (ARF) transcription factor family regulates gene expression in response to auxin. In the absence of auxin, ARF transcription factors are repressed by interaction with AUXIN/INDOLE 3-ACETIC ACID (Aux/IAA) proteins. Although the C termini of ARF and Aux/IAA proteins facilitate their homo- and heterooligomerization, the molecular basis for this interaction remained undefined. The crystal structure of the C-terminal interaction domain of Arabidopsis ARF7 reveals a Phox and Bem1p (PB1) domain that provides both positive and negative electrostatic interfaces for directional protein interaction. Mutation of interface residues in the ARF7 PB1 domain yields monomeric protein and abolishes interaction with both itself and IAA17. Expression of a stabilized Aux/IAA protein (i.e., IAA16) bearing PB1 mutations in Arabidopsis suggests a multimerization requirement for ARF protein repression, leading to a refined auxin-signaling model.
Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression.,Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32. doi:, 10.1073/pnas.1400074111. Epub 2014 Mar 25. PMID:24706860[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hagen G, Guilfoyle T. Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol Biol. 2002 Jun-Jul;49(3-4):373-85. PMID:12036261
- ↑ Hardtke CS, Ckurshumova W, Vidaurre DP, Singh SA, Stamatiou G, Tiwari SB, Hagen G, Guilfoyle TJ, Berleth T. Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4. Development. 2004 Mar;131(5):1089-100. PMID:14973283 doi:http://dx.doi.org/10.1242/dev.00925
- ↑ Li J, Dai X, Zhao Y. A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis. Plant Physiol. 2006 Mar;140(3):899-908. Epub 2006 Feb 3. PMID:16461383 doi:http://dx.doi.org/pp.105.070987
- ↑ Okushima Y, Fukaki H, Onoda M, Theologis A, Tasaka M. ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis. Plant Cell. 2007 Jan;19(1):118-30. Epub 2007 Jan 26. PMID:17259263 doi:http://dx.doi.org/tpc.106.047761
- ↑ Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC. Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32. doi:, 10.1073/pnas.1400074111. Epub 2014 Mar 25. PMID:24706860 doi:http://dx.doi.org/10.1073/pnas.1400074111
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