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| | ==CSL (RBP-Jk) with corepressor KyoT2 bound to DNA== | | ==CSL (RBP-Jk) with corepressor KyoT2 bound to DNA== |
| - | <StructureSection load='4j2x' size='340' side='right' caption='[[4j2x]], [[Resolution|resolution]] 2.85Å' scene=''> | + | <StructureSection load='4j2x' size='340' side='right'caption='[[4j2x]], [[Resolution|resolution]] 2.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j2x]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J2X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j2x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J2X FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ttu|1ttu]], [[2fo1|2fo1]], [[3brd|3brd]], [[3brf|3brf]], [[3brg|3brg]], [[3iag|3iag]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Igkjrb1, Igkrsbp, RBP-Jk, Rbpj, Rbpsuh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Fhl1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2x OCA], [https://pdbe.org/4j2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j2x RCSB], [https://www.ebi.ac.uk/pdbsum/4j2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2x ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2x OCA], [http://pdbe.org/4j2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j2x RCSB], [http://www.ebi.ac.uk/pdbsum/4j2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2x ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SUH_MOUSE SUH_MOUSE]] Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.<ref>PMID:7566092</ref> [[http://www.uniprot.org/uniprot/FHL1_MOUSE FHL1_MOUSE]] May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems. | + | [https://www.uniprot.org/uniprot/SUH_MOUSE SUH_MOUSE] Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.<ref>PMID:7566092</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Collins, K J]] | + | [[Category: Large Structures]] |
| - | [[Category: Kovall, R A]] | + | [[Category: Mus musculus]] |
| - | [[Category: Dna binding]] | + | [[Category: Collins KJ]] |
| - | [[Category: Lim domain containing protein]] | + | [[Category: Kovall RA]] |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Transcription factor corepressor]]
| + | |
| - | [[Category: Transcription-dna binding protein-dna complex]]
| + | |
| Structural highlights
Function
SUH_MOUSE Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.[1]
Publication Abstract from PubMed
Notch refers to a highly conserved cell-to-cell signaling pathway with essential roles in embryonic development and tissue maintenance. Dysfunctional signaling causes human disease, highlighting the importance of pathway regulation. Notch signaling ultimately results in the activation of target genes, which is regulated by the nuclear effector CSL (CBF-1/RBP-J, Su(H), Lag-1). CSL dually functions as an activator and a repressor of transcription through differential interactions with coactivator or corepressor proteins, respectively. Although the structures of CSL-coactivator complexes have been determined, the structures of CSL-corepressor complexes are unknown. Here, using a combination of structural, biophysical, and cellular approaches, we characterize the structure and function of CSL in complex with the corepressor KyoT2. Collectively, our studies provide molecular insights into how KyoT2 binds CSL with high affinity and competes with coactivators, such as Notch, for binding CSL. These studies are important for understanding how CSL functions as both an activator and a repressor of transcription of Notch target genes.
Structure and Function of the CSL-KyoT2 Corepressor Complex: A Negative Regulator of Notch Signaling.,Collins KJ, Yuan Z, Kovall RA Structure. 2014 Jan 7;22(1):70-81. doi: 10.1016/j.str.2013.10.010. Epub 2013 Nov , 27. PMID:24290140[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jarriault S, Brou C, Logeat F, Schroeter EH, Kopan R, Israel A. Signalling downstream of activated mammalian Notch. Nature. 1995 Sep 28;377(6547):355-8. PMID:7566092 doi:http://dx.doi.org/10.1038/377355a0
- ↑ Collins KJ, Yuan Z, Kovall RA. Structure and Function of the CSL-KyoT2 Corepressor Complex: A Negative Regulator of Notch Signaling. Structure. 2014 Jan 7;22(1):70-81. doi: 10.1016/j.str.2013.10.010. Epub 2013 Nov , 27. PMID:24290140 doi:http://dx.doi.org/10.1016/j.str.2013.10.010
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