4pmr

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the Mycobacterium tuberculosis Tat-secreted protein Rv2525c in complex with HEPES (monoclinic crystal form II)

Structural highlights

4pmr is a 1 chain structure with sequence from Mycobacterium tuberculosis CDC1551. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.81Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P95028_MYCTO

Publication Abstract from PubMed

Among the few proteins shown to be secreted by the Tat system in Mycobacterium tuberculosis, Rv2525c is of particular interest, since its gene is conserved in the minimal genome of Mycobacterium leprae. Previous evidence linked this protein to cell wall metabolism and sensitivity to beta-lactams. We describe here the crystal structure of Rv2525c that shows a TIM barrel-like fold characteristic of glycoside hydrolases of the GH25 family, which includes prokaryotic and phage-encoded peptidoglycan hydrolases. Structural comparison with other members of this family combined with substrate docking suggest that, although the 'neighbouring group' catalytic mechanism proposed for this family still appears as the most plausible, the identity of residues involved in catalysis in GH25 hydrolases might need to be revised.

Structural studies suggest a peptidoglycan hydrolase function for the Mycobacterium tuberculosis Tat-secreted protein Rv2525c.,Bellinzoni M, Haouz A, Miras I, Magnet S, Andre-Leroux G, Mukherjee R, Shepard W, Cole ST, Alzari PM J Struct Biol. 2014 Sep 24. pii: S1047-8477(14)00189-0. doi:, 10.1016/j.jsb.2014.09.003. PMID:25260828^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bellinzoni M, Haouz A, Miras I, Magnet S, Andre-Leroux G, Mukherjee R, Shepard W, Cole ST, Alzari PM. Structural studies suggest a peptidoglycan hydrolase function for the Mycobacterium tuberculosis Tat-secreted protein Rv2525c. J Struct Biol. 2014 Sep 24. pii: S1047-8477(14)00189-0. doi:, 10.1016/j.jsb.2014.09.003. PMID:25260828 doi:http://dx.doi.org/10.1016/j.jsb.2014.09.003

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pmr"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Mycobacterium tuberculosis Tat-secreted protein Rv2525c in complex with HEPES (monoclinic crystal form II)==
-<StructureSection load='4pmr' size='340' side='right' caption='[[4pmr]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
+<StructureSection load='4pmr' size='340' side='right'caption='[[4pmr]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4pmr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PMR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4pmr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_CDC1551 Mycobacterium tuberculosis CDC1551]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PMR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pmr OCA], [http://pdbe.org/4pmr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pmr RCSB], [http://www.ebi.ac.uk/pdbsum/4pmr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pmr ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pmr OCA], [https://pdbe.org/4pmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pmr RCSB], [https://www.ebi.ac.uk/pdbsum/4pmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pmr ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/P95028_MYCTO P95028_MYCTO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Alzari, P M]]
+[[Category: Large Structures]]
-[[Category: Bellinzoni, M]]
+[[Category: Mycobacterium tuberculosis CDC1551]]
-[[Category: Haouz, A]]
+[[Category: Alzari PM]]
-[[Category: Shepard, W]]
+[[Category: Bellinzoni M]]
-[[Category: Unknown function]]
+[[Category: Haouz A]]
+[[Category: Shepard W]]

4pmr

From Proteopedia

Current revision

Crystal structure of the Mycobacterium tuberculosis Tat-secreted protein Rv2525c in complex with HEPES (monoclinic crystal form II)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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