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| | ==Structural basis for catalytic activation of a serine recombinase== | | ==Structural basis for catalytic activation of a serine recombinase== |
| - | <StructureSection load='3pkz' size='340' side='right' caption='[[3pkz]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3pkz' size='340' side='right'caption='[[3pkz]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3pkz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PKZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3pkz]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PKZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2r0q|2r0q]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sin, SAP013A_018, SAP095A_006, SAP096A_020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pkz OCA], [https://pdbe.org/3pkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pkz RCSB], [https://www.ebi.ac.uk/pdbsum/3pkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pkz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pkz OCA], [http://pdbe.org/3pkz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pkz RCSB], [http://www.ebi.ac.uk/pdbsum/3pkz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pkz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/BIN3_STAAU BIN3_STAAU] Potential DNA invertase. |
| - | Sin resolvase is a site-specific serine recombinase that is normally controlled by a complex regulatory mechanism. A single mutation, Q115R, allows the enzyme to bypass the entire regulatory apparatus, such that no accessory proteins or DNA sites are required. Here, we present a 1.86 A crystal structure of the Sin Q115R catalytic domain, in a tetrameric arrangement stabilized by an interaction between Arg115 residues on neighboring subunits. The subunits have undergone significant conformational changes from the inactive dimeric state previously reported. The structure provides a new high-resolution view of a serine recombinase active site that is apparently fully assembled, suggesting roles for the conserved active site residues. The structure also suggests how the dimer-tetramer transition is coupled to assembly of the active site. The tetramer is captured in a different rotational substate than that seen in previous hyperactive serine recombinase structures, and unbroken crossover site DNA can be readily modeled into its active sites.
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| - | Structural basis for catalytic activation of a serine recombinase.,Keenholtz RA, Rowland SJ, Boocock MR, Stark WM, Rice PA Structure. 2011 Jun 8;19(6):799-809. PMID:21645851<ref>PMID:21645851</ref>
| + | ==See Also== |
| - | | + | *[[Resolvase 3D structures|Resolvase 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3pkz" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Boocock, M R]] | + | [[Category: Large Structures]] |
| - | [[Category: Keenholtz, R A]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Rice, P A]] | + | [[Category: Boocock MR]] |
| - | [[Category: Rowland, S J]] | + | [[Category: Keenholtz RA]] |
| - | [[Category: Stark, W M]] | + | [[Category: Rice PA]] |
| - | [[Category: Dna]] | + | [[Category: Rowland SJ]] |
| - | [[Category: Recombination]] | + | [[Category: Stark WM]] |
| - | [[Category: Resolvase]]
| + | |
| - | [[Category: Small serine recombinase]]
| + | |
