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| | ==Anti Rotavirus Antibody== | | ==Anti Rotavirus Antibody== |
| - | <StructureSection load='4hfw' size='340' side='right' caption='[[4hfw]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='4hfw' size='340' side='right'caption='[[4hfw]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hfw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hfw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HFW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfw OCA], [http://pdbe.org/4hfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hfw RCSB], [http://www.ebi.ac.uk/pdbsum/4hfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfw ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfw OCA], [https://pdbe.org/4hfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hfw RCSB], [https://www.ebi.ac.uk/pdbsum/4hfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Aiyegbo, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Crowe, J E]] | + | [[Category: Aiyegbo M]] |
| - | [[Category: Spiller, B W]] | + | [[Category: Crowe JE]] |
| - | [[Category: Ig fold]] | + | [[Category: Spiller BW]] |
| - | [[Category: Immune response]]
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| - | [[Category: Immune system]]
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| - | [[Category: Rotavirus vp6 protein]]
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| Structural highlights
Publication Abstract from PubMed
Several live attenuated rotavirus (RV) vaccines have been licensed, but the mechanisms of protective immunity are still poorly understood. The most frequent human B cell response is directed to the internal protein VP6 on the surface of double-layered particles, which is normally exposed only in the intracellular environment. Here, we show that the canonical VP6 antibodies secreted by humans bind to such particles and inhibit viral transcription. Polymeric IgA RV antibodies mediated an inhibitory effect against virus replication inside cells during IgA transcytosis. We defined the recognition site on VP6 as a quaternary epitope containing a high density of charged residues. RV human mAbs appear to bind to a negatively-charged patch on the surface of the Type I channel in the transcriptionally active particle, and they sterically block the channel. This unique mucosal mechanism of viral neutralization, which is not apparent from conventional immunoassays, may contribute significantly to human immunity to RV.
Human Rotavirus VP6-Specific Antibodies Mediate Intracellular Neutralization by Binding to a Quaternary Structure in the Transcriptional Pore.,Aiyegbo MS, Sapparapu G, Spiller BW, Eli IM, Williams DR, Kim R, Lee DE, Liu T, Li S, Woods VL Jr, Nannemann DP, Meiler J, Stewart PL, Crowe JE Jr PLoS One. 2013 May 9;8(5):e61101. doi: 10.1371/journal.pone.0061101. Print 2013. PMID:23671563[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Aiyegbo MS, Sapparapu G, Spiller BW, Eli IM, Williams DR, Kim R, Lee DE, Liu T, Li S, Woods VL Jr, Nannemann DP, Meiler J, Stewart PL, Crowe JE Jr. Human Rotavirus VP6-Specific Antibodies Mediate Intracellular Neutralization by Binding to a Quaternary Structure in the Transcriptional Pore. PLoS One. 2013 May 9;8(5):e61101. doi: 10.1371/journal.pone.0061101. Print 2013. PMID:23671563 doi:10.1371/journal.pone.0061101
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