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| | ==Crystal structure of ScpAB core complex== | | ==Crystal structure of ScpAB core complex== |
| - | <StructureSection load='3w6j' size='340' side='right' caption='[[3w6j]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='3w6j' size='340' side='right'caption='[[3w6j]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3w6j]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W6J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W6J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3w6j]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W6J FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w6k|3w6k]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980]), scpB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w6j OCA], [https://pdbe.org/3w6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w6j RCSB], [https://www.ebi.ac.uk/pdbsum/3w6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w6j ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w6j OCA], [http://pdbe.org/3w6j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w6j RCSB], [http://www.ebi.ac.uk/pdbsum/3w6j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w6j ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0E0TD69_GEOS2 A0A0E0TD69_GEOS2] Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves.[HAMAP-Rule:MF_01805] |
| - | In many bacteria, a homodimer of structural-maintenance-of-chromosomes proteins associates with two regulatory subunits (known as ScpA and ScpB), assembling a protein complex that plays a crucial role in chromosome organization and segregation. It remains poorly understood, however, how this complex might work at the mechanistic level. Here, we report crystal structures of the ScpAB core complex that display a highly unusual structure in which the central segment of ScpA winds around an asymmetrically oriented ScpB dimer. The two C-terminal domains of the ScpB dimer primarily interact with different regions of ScpA with different affinities. Moreover, flexible interdomain regions of ScpB contribute to a dynamic folding process of the ScpAB subcomplex. Together with other genetic and biochemical assays, we provide evidence that internal structural changes of the ScpAB subcomplex are tightly coupled with activation of the structural-maintenance-of-chromosomes ATPase.
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| - | Molecular Basis of SMC ATPase Activation: Role of Internal Structural Changes of the Regulatory Subcomplex ScpAB.,Kamada K, Miyata M, Hirano T Structure. 2013 Mar 26. pii: S0969-2126(13)00057-9. doi:, 10.1016/j.str.2013.02.016. PMID:23541893<ref>PMID:23541893</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 3w6j" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 12980]] | + | [[Category: Geobacillus stearothermophilus]] |
| - | [[Category: Hirano, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Kamada, K]] | + | [[Category: Hirano T]] |
| - | [[Category: Cell cycle]] | + | [[Category: Kamada K]] |
| - | [[Category: Regulatory subcomplex]]
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| - | [[Category: Smc]]
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| - | [[Category: Winged hth]]
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