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1kor

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[[Image:1kor.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with inhibitors==
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|PDB= 1kor |SIZE=350|CAPTION= <scene name='initialview01'>1kor</scene>, resolution 1.95&Aring;
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<StructureSection load='1kor' size='340' side='right'caption='[[1kor]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>
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<table><tr><td colspan='2'>[[1kor]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KOR FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_synthase Argininosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.5 6.3.4.5] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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|GENE= HB8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kor OCA], [https://pdbe.org/1kor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kor RCSB], [https://www.ebi.ac.uk/pdbsum/1kor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kor ProSAT], [https://www.topsan.org/Proteins/RSGI/1kor TOPSAN]</span></td></tr>
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|RELATEDENTRY=[[1kh1|1KH1]], [[1kh2|1KH2]], [[1kh3|1KH3]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kor OCA], [http://www.ebi.ac.uk/pdbsum/1kor PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kor RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ASSY_THET8 ASSY_THET8]
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== Evolutionary Conservation ==
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'''Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with inhibitors'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/1kor_consurf.spt"</scriptWhenChecked>
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Argininosuccinate synthetase catalyzes the ATP-dependent condensation of a citrulline with an aspartate to give argininosuccinate. The three-dimensional structures of the enzyme from Thermus thermophilus HB8 in its free form, complexed with intact ATP, and complexed with an ATP analogue (adenylyl imidodiphosphate) and substrate analogues (arginine and succinate) have been determined at 2.3-, 2.3-, and 1.95-A resolution, respectively. The structure is essentially the same as that of the Escherichia coli argininosuccinate synthetase. The small domain has the same fold as that of a new family of "N-type" ATP pyrophosphatases with the P-loop specific for the pyrophosphate of ATP. However, the enzyme shows the P-loop specific for the gamma-phosphate of ATP. The structure of the complex form is quite similar to that of the native one, indicating that no conformational change occurs upon the binding of ATP and the substrate analogues. ATP and the substrate analogues are bound to the active site with their reaction sites close to one another and located in a geometrical orientation favorable to the catalytic action. The reaction mechanism so far proposed seems to be consistent with the locations of ATP and the substrate analogues. The reaction may proceed without the large conformational change of the enzyme proposed for the catalytic process.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1KOR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOR OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kor ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action., Goto M, Nakajima Y, Hirotsu K, J Biol Chem. 2002 May 3;277(18):15890-6. Epub 2002 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11844799 11844799]
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</StructureSection>
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[[Category: Argininosuccinate synthase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Goto, M.]]
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[[Category: Goto M]]
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[[Category: Hirotsu, K.]]
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[[Category: Hirotsu K]]
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[[Category: Nakajima, Y.]]
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[[Category: Nakajima Y]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: ligase]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:51:28 2008''
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Current revision

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with inhibitors

PDB ID 1kor

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