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| | ==Crystal structure of the antibody-binding region of Protein M (Protein M TD) in complex with anti-HIV antibody PGT135 Fab== | | ==Crystal structure of the antibody-binding region of Protein M (Protein M TD) in complex with anti-HIV antibody PGT135 Fab== |
| - | <StructureSection load='4nzr' size='340' side='right' caption='[[4nzr]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='4nzr' size='340' side='right'caption='[[4nzr]], [[Resolution|resolution]] 1.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4nzr]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Mycge Mycge]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NZR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4nzr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mycoplasma_genitalium_G37 Mycoplasma genitalium G37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NZR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jm4|4jm4]], [[4nzt|4nzt]], [[4nzu|4nzu]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MG281 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243273 MYCGE])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nzr OCA], [https://pdbe.org/4nzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nzr RCSB], [https://www.ebi.ac.uk/pdbsum/4nzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nzr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nzr OCA], [http://pdbe.org/4nzr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nzr RCSB], [http://www.ebi.ac.uk/pdbsum/4nzr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nzr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Y281_MYCGE Y281_MYCGE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Mycge]] | + | [[Category: Large Structures]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Mycoplasma genitalium G37]] |
| - | [[Category: Zhu, X]] | + | [[Category: Wilson IA]] |
| - | [[Category: Block antibody-antigen union]] | + | [[Category: Zhu X]] |
| - | [[Category: Broad antibody-binding]]
| + | |
| - | [[Category: Immune system-protein binding complex]]
| + | |
| - | [[Category: Leucine-rich repeat]]
| + | |
| - | [[Category: Variable region]]
| + | |
| Structural highlights
Function
Y281_MYCGE
Publication Abstract from PubMed
We report the discovery of a broadly reactive antibody-binding protein (Protein M) from human mycoplasma. The crystal structure of the ectodomain of transmembrane Protein M differs from other known protein structures, as does its mechanism of antibody binding. Protein M binds with high affinity to all types of human and nonhuman immunoglobulin G, predominantly through attachment to the conserved portions of the variable region of the kappa and lambda light chains. Protein M blocks antibody-antigen union, likely because of its large C-terminal domain extending over the antibody-combining site, blocking entry to large antigens. Similar to the other immunoglobulin-binding proteins such as Protein A, Protein M as well as its orthologs in other Mycoplasma species could become invaluable reagents in the antibody field.
A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.,Grover RK, Zhu X, Nieusma T, Jones T, Boero I, MacLeod AS, Mark A, Niessen S, Kim HJ, Kong L, Assad-Garcia N, Kwon K, Chesi M, Smider VV, Salomon DR, Jelinek DF, Kyle RA, Pyles RB, Glass JI, Ward AB, Wilson IA, Lerner RA Science. 2014 Feb 7;343(6171):656-61. doi: 10.1126/science.1246135. PMID:24503852[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grover RK, Zhu X, Nieusma T, Jones T, Boero I, MacLeod AS, Mark A, Niessen S, Kim HJ, Kong L, Assad-Garcia N, Kwon K, Chesi M, Smider VV, Salomon DR, Jelinek DF, Kyle RA, Pyles RB, Glass JI, Ward AB, Wilson IA, Lerner RA. A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union. Science. 2014 Feb 7;343(6171):656-61. doi: 10.1126/science.1246135. PMID:24503852 doi:http://dx.doi.org/10.1126/science.1246135
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