|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of DCC, a netrin-1 receptor== | | ==Structure of DCC, a netrin-1 receptor== |
| - | <StructureSection load='3laf' size='340' side='right' caption='[[3laf]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3laf' size='340' side='right'caption='[[3laf]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3laf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LAF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3laf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LAF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dcc, rCG_46581 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3laf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3laf OCA], [http://pdbe.org/3laf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3laf RCSB], [http://www.ebi.ac.uk/pdbsum/3laf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3laf ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3laf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3laf OCA], [https://pdbe.org/3laf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3laf RCSB], [https://www.ebi.ac.uk/pdbsum/3laf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3laf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DCC_RAT DCC_RAT]] Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene (By similarity).<ref>PMID:8861902</ref> | + | [https://www.uniprot.org/uniprot/DCC_RAT DCC_RAT] Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene (By similarity).<ref>PMID:8861902</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 26: |
Line 26: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Large Structures]] |
| - | [[Category: Chen, Q]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Liu, J H]] | + | [[Category: Chen Q]] |
| - | [[Category: Wang, J H]] | + | [[Category: Liu J-H]] |
| - | [[Category: Apoptosis]] | + | [[Category: Wang J-H]] |
| - | [[Category: Horseshoe]]
| + | |
| - | [[Category: Immunoglobulin superfamily]]
| + | |
| - | [[Category: Netrin-1 receptor]]
| + | |
| Structural highlights
Function
DCC_RAT Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene (By similarity).[1]
Publication Abstract from PubMed
Deleted in colorectal cancer (DCC) is a receptor for the axon guidance cues netrin-1 and draxin. The interactions between these guidance cues and DCC play a key role in the development of the nervous system. In the present study, we reveal the crystal structure of the N-terminal four Ig-like domains of DCC. The molecule folds into a horseshoe-like configuration. We demonstrate that this horseshoe conformation of DCC is required for guidance-cue-mediated axonal attraction. Structure-based mutations that disrupt the DCC horseshoe indeed impair its function. A comparison of the DCC horseshoe with previously described horseshoe structures has revealed striking conserved structural features and important sequence signatures. Using these signatures, a genome-wide search allows us to predict the N-terminal horseshoe arrangement in a number of other cell surface receptors, nearly all of which function in the nervous system. The N-terminal horseshoe appears to be evolutionally selected as a platform for neural receptors.
N-terminal horseshoe conformation of DCC is functionally required for axon guidance and might be shared by other neural receptors.,Chen Q, Sun X, Zhou XH, Liu JH, Wu J, Zhang Y, Wang JH J Cell Sci. 2013 Jan 1;126(Pt 1):186-95. doi: 10.1242/jcs.111278. Epub 2012 Oct, 4. PMID:23038776[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Keino-Masu K, Masu M, Hinck L, Leonardo ED, Chan SS, Culotti JG, Tessier-Lavigne M. Deleted in Colorectal Cancer (DCC) encodes a netrin receptor. Cell. 1996 Oct 18;87(2):175-85. PMID:8861902
- ↑ Chen Q, Sun X, Zhou XH, Liu JH, Wu J, Zhang Y, Wang JH. N-terminal horseshoe conformation of DCC is functionally required for axon guidance and might be shared by other neural receptors. J Cell Sci. 2013 Jan 1;126(Pt 1):186-95. doi: 10.1242/jcs.111278. Epub 2012 Oct, 4. PMID:23038776 doi:http://dx.doi.org/10.1242/jcs.111278
|
