3nio

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Pseudomonas aeruginosa guanidinobutyrase

Structural highlights

3nio is a 6 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GBUA_PSEAE Catalyzes specifically the hydrolysis of 4-guanidinobutanoate to 4-aminobutanoate and urea. Has no activity against arginine, agmatine, 3-guanidinopropionate and guanidinoacetate.^[1] ^[2]

Publication Abstract from PubMed

Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) catalyze the hydrolysis of 4-guanidinobutyrate and 3-guanidinopropionate, respectively. They belong to the ureohydrolase superfamily, which includes arginase, agmatinase, proclavaminate amidinohydrolase, and formiminoglutamase. In this study, we have determined the crystal structures of GbuA and GpuA from P. aeruginosa to provide a structural insight into their substrate specificity. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops. Mutagenesis of Met161 of GbuA and Tyr157 of GpuA, both of which are located in the active site loop 1 and predicted to be involved in substrate recognition, significantly affected their enzymatic properties, implying their important roles in catalysis.

Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily.,Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW J Struct Biol. 2011 May 12. PMID:21600989^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nakada Y, Itoh Y. Characterization and regulation of the gbuA gene, encoding guanidinobutyrase in the arginine dehydrogenase pathway of Pseudomonas aeruginosa PAO1. J Bacteriol. 2002 Jun;184(12):3377-84. PMID:12029055 doi:10.1128/JB.184.12.3377-3384.2002
↑ Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW. Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol. 2011 May 12. PMID:21600989 doi:10.1016/j.jsb.2011.05.002
↑ Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW. Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol. 2011 May 12. PMID:21600989 doi:10.1016/j.jsb.2011.05.002

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nio"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Pseudomonas aeruginosa guanidinobutyrase==
-<StructureSection load='3nio' size='340' side='right' caption='[[3nio]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='3nio' size='340' side='right'caption='[[3nio]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nio]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NIO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nio]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NIO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nip|3nip]], [[3niq|3niq]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nio OCA], [https://pdbe.org/3nio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nio RCSB], [https://www.ebi.ac.uk/pdbsum/3nio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nio ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gbuA, PA1421 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Guanidinobutyrase Guanidinobutyrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.7 3.5.3.7] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nio OCA], [http://pdbe.org/3nio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nio RCSB], [http://www.ebi.ac.uk/pdbsum/3nio PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nio ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GBUA_PSEAE GBUA_PSEAE] Catalyzes specifically the hydrolysis of 4-guanidinobutanoate to 4-aminobutanoate and urea. Has no activity against arginine, agmatine, 3-guanidinopropionate and guanidinoacetate.<ref>PMID:12029055</ref> <ref>PMID:21600989</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Guanidinobutyrase]]
+[[Category: Large Structures]]
-[[Category: Pseae]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: An, D]]
+[[Category: An D]]
-[[Category: Im, H]]
+[[Category: Im H]]
-[[Category: Jang, J Y]]
+[[Category: Jang JY]]
-[[Category: Kim, D J]]
+[[Category: Kim DJ]]
-[[Category: Kim, H S]]
+[[Category: Kim HS]]
-[[Category: Kim, K H]]
+[[Category: Kim KH]]
-[[Category: Lee, S J]]
+[[Category: Lee SJ]]
-[[Category: Suh, S W]]
+[[Category: Suh SW]]
-[[Category: Yoon, H J]]
+[[Category: Yoon HJ]]
-[[Category: Yoon, J Y]]
+[[Category: Yoon JY]]
-[[Category: Gbua]]
-[[Category: Hydrolase]]
-[[Category: Pa1421]]

3nio

From Proteopedia

Current revision

Crystal structure of Pseudomonas aeruginosa guanidinobutyrase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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