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4g9p

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==Structure of the GcpE-MEcPP (IspG) complex from Thermus thermophilus==
==Structure of the GcpE-MEcPP (IspG) complex from Thermus thermophilus==
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<StructureSection load='4g9p' size='340' side='right' caption='[[4g9p]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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<StructureSection load='4g9p' size='340' side='right'caption='[[4g9p]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4g9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G9P FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4g9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G9P FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CDI:2C-METHYL-D-ERYTHRITOL+2,4-CYCLODIPHOSPHATE'>CDI</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gcpE, ispG, TT_C1677 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDI:2C-METHYL-D-ERYTHRITOL+2,4-CYCLODIPHOSPHATE'>CDI</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate_synthase (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.7.1 1.17.7.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9p OCA], [https://pdbe.org/4g9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g9p RCSB], [https://www.ebi.ac.uk/pdbsum/4g9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g9p ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9p OCA], [http://pdbe.org/4g9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g9p RCSB], [http://www.ebi.ac.uk/pdbsum/4g9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g9p ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/ISPG_THET2 ISPG_THET2]
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Isoprenoid precursor biosynthesis occurs through the mevalonate or the methylerythritol phosphate (MEP) pathway, used i.e., by humans and by many human pathogens, respectively. In the MEP pathway, 2-C-methyl-d-erythritol-2,4-cyclo-diphosphate (MEcPP) is converted to (E)-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate (HMBPP) by the iron-sulfur cluster enzyme HMBPP synthase (GcpE). The presented X-ray structure of the GcpE-MEcPP complex from Thermus thermophilus at 1.55A resolution provides valuable information about the catalytic mechanism and for rational inhibitor design. MEcPP binding inside the TIM-barrel funnel induces a 60 degrees rotation of the [4Fe-4S] cluster containing domain onto the TIM-barrel entrance. The apical iron of the [4Fe-4S] cluster ligates with the C3 oxygen atom of MEcPP.
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Structure of the GcpE (IspG)-MEcPP complex from Thermus thermophilus.,Rekittke I, Jomaa H, Ermler U FEBS Lett. 2012 Sep 21;586(19):3452-7. doi: 10.1016/j.febslet.2012.07.070. Epub, 2012 Aug 9. PMID:22967895<ref>PMID:22967895</ref>
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==See Also==
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*[[IspG|IspG]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4g9p" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Thet2]]
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[[Category: Large Structures]]
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[[Category: Ermler, U]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Jomaa, H]]
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[[Category: Ermler U]]
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[[Category: Rekittke, I]]
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[[Category: Jomaa H]]
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[[Category: Iron-sulphur-cluster]]
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[[Category: Rekittke I]]
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[[Category: Isoprenoid biosynthesis]]
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[[Category: Mecpp]]
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[[Category: Non mevalonate pathway]]
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[[Category: Oxidoreductase]]
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[[Category: Tim-barrel]]
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Current revision

Structure of the GcpE-MEcPP (IspG) complex from Thermus thermophilus

PDB ID 4g9p

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