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| | ==3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium== | | ==3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium== |
| - | <StructureSection load='4m64' size='340' side='right' caption='[[4m64]], [[Resolution|resolution]] 3.35Å' scene=''> | + | <StructureSection load='4m64' size='340' side='right'caption='[[4m64]], [[Resolution|resolution]] 3.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4m64]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M64 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M64 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4m64]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M64 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">melB, STM4299 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m64 OCA], [http://pdbe.org/4m64 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m64 RCSB], [http://www.ebi.ac.uk/pdbsum/4m64 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m64 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m64 OCA], [https://pdbe.org/4m64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m64 RCSB], [https://www.ebi.ac.uk/pdbsum/4m64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m64 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MELB_SALTY MELB_SALTY]] Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system). | + | [https://www.uniprot.org/uniprot/MELB_SALTY MELB_SALTY] Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system). |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The bacterial melibiose permease (MelB) belongs to the glycoside-pentoside-hexuronide:cation symporter family, a part of the major facilitator superfamily (MFS). Structural information regarding glycoside-pentoside-hexuronide:cation symporter family transporters and other Na(+)-coupled permeases within MFS has been lacking, although a wealth of biochemical and biophysical data are available. Here we present the three-dimensional crystal structures of Salmonella typhimurium MelBSt in two conformations, representing an outward partially occluded and an outward inactive state of MelBSt. MelB adopts a typical MFS fold and contains a previously unidentified cation-binding motif. Three conserved acidic residues form a pyramidal-shaped cation-binding site for Na(+), Li(+) or H(+), which is in close proximity to the sugar-binding site. Both cosubstrate-binding sites are mainly contributed by the residues from the amino-terminal domain. These two structures and the functional data presented here provide mechanistic insights into Na(+)/melibiose symport. We also postulate a structural foundation for the conformational cycling necessary for transport catalysed by MFS permeases in general.
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| - | Structure-based mechanism for Na(+)/melibiose symport by MelB.,Ethayathulla AS, Yousef MS, Amin A, Leblanc G, Kaback HR, Guan L Nat Commun. 2014 Jan 6;5:3009. doi: 10.1038/ncomms4009. PMID:24389923<ref>PMID:24389923</ref>
| + | ==See Also== |
| - | | + | *[[Symporter 3D structures|Symporter 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4m64" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Salty]] | + | [[Category: Large Structures]] |
| - | [[Category: Ethayathulla, A S]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] |
| - | [[Category: Guan, L]] | + | [[Category: Ethayathulla AS]] |
| - | [[Category: Glycoside-pentoside-hexuronide:cation symporter family]] | + | [[Category: Guan L]] |
| - | [[Category: Major facilitator superfamily]]
| + | |
| - | [[Category: Melibiose permease]]
| + | |
| - | [[Category: Melibiose/na+ symport]]
| + | |
| - | [[Category: Membrane carrier]]
| + | |
| - | [[Category: Membrane transport protein]]
| + | |
| - | [[Category: Secondary active transport]]
| + | |
| - | [[Category: Transport protein]]
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